merck millipore,默克密理博,07-1413,Anti-GSK-3b Antibody, a.a. 335-349

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merck millipore,默克密理博,07-1413,Anti-GSK-3b Antibody, a.a. 335-349
产品名称：Anti-GSK-3b Antibody, a.a. 335-349
产品型号：07-1413
This AntiI-GSK-3b Antibody, a.a. 335-349 is validated for use in WB, IP, IH(P) for the detection of AntiI-GSK-3b.

merck millipore,默克密理博,07-1413,Anti-GSK-3b Antibody, a.a. 335-349

产品介绍

merck millipore,默克密理博,07-1413,Anti-GSK-3b Antibody, a.a. 335-349

Replacement Information
Replacement Information	07-1413 is a recommended replacement for AB8687

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
B, R, H, M	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-1413
Replaces	AB8687
描述	Anti-GSK-3b Antibody, a.a. 335-349
背景信息	GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.

产品信息
格式	Affinity Purified
控制	C2C12 Cell Lysate, Hela cells, rat or mouse brain
演示	Purified polyclonal IgG antibody is supplied in PBS, pH 7.2, 50% glycerol and 0.09% sodium azide.

应用
应用	This AntiI-GSK-3b Antibody, a.a. 335-349 is validated for use in WB, IP, IH(P) for the detection of AntiI-GSK-3b.
主要应用	Western Blotting
应用说明	Immunoprecipitation: 12.5 µg/mL dilution of a previous lot was ued in immunoprecipitation on rat brain tissue; immunoprecipitations with tissue extracts other than brain may require higher antibody concentrations. Optimal working dilutions must be determined by the end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of GSK3β Polyclonal Antibody: HeLa Cells

生物信息
免疫原品种	Rat GSK3 beta synthetic peptide (aa. 335-349), conjugated to KLH. This sequence is 100% identical to human GSK3 beta.
表位	a.a. 335-349
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody detects a strong 47 kDa protein, corresponding to the expected molecular mass of GSK3 beta.
同种型	IgG
品种反应性	BovineRatHumanMouse
Species Reactivity Note	Rat, Human, Mouse and Bovine
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_002093
Entrez基因汇总	Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and nactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed1333807]).[supplied by OMIM].
基因符号	EC 2.7.11.26 GSK3B
纯化方法	Protein A affinity purified
UniProt编号	P49841
UniProt汇总	FUNCTION: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate. ENZYME REGULATION: Inhibited when phosphorylated by AKT1. SUBUNIT: Monomer (By similarity). Interacts with CABYR, MUC1, NIN and PRUNE. ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

产品使用声明
质量保证	Routinely evaluated by Western Blot on C2C12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected GSK3 BETA on 10 μg of C2C12 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable at –20 ºC for up to one year from date of receipt. Product may be stored at for 2-4 weeks at 2º-8ºC. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable at –20 ºC for up to one year from date of receipt. Product may be stored at for 2-4 weeks at 2º-8ºC.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.