merck millipore,默克密理博,07-1382,Anti-eEF2 (CT) Antibody

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merck millipore,默克密理博,07-1382,Anti-eEF2 (CT) Antibody
产品名称：Anti-eEF2 (CT) Antibody
产品型号：07-1382
This Anti-eEF2 (C-term) Antibody is validated for use in WB, IC, IH, FC, IP for the detection of eEF2 (C-term).

merck millipore,默克密理博,07-1382,Anti-eEF2 (CT) Antibody

产品介绍

merck millipore,默克密理博,07-1382,Anti-eEF2 (CT) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB, ICC, IHC	Rb	Unpurified	Polyclonal Antibody

描述
产品目录编号	07-1382
描述	Anti-eEF2 (CT) Antibody
Alternate Names	polypeptidyl-tRNA translocase eukaryotic translation elongation factor 2
背景信息	eEF2 (eukaryotic translation elongation factor 2) is essential for protein synthesis. It promotes the GTP-dependent translocation during peptide chain elongation. eEF2 is phosphorylated and inhibited by eEF2 kinase (eEF2K) on Thr56. eEF2K activity is inhibited by phosphorylation by both mTOR and p70S6K. This in turn up regulates eEF2 activity and protein synthesis. Similarly, AMPK down regulates protein synthesis by inhibited the activity of both p70S6K and mTOR, through TSC2.

产品信息
格式	Unpurified
控制	HEK293 cell lysate.
演示	Unpurified rabbit polyclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	This Anti-eEF2 (C-term) Antibody is validated for use in WB, IC, IH, FC, IP for the detection of eEF2 (C-term).
主要应用	Flow Cytometry Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Immunohistochemistry: 1:100 – 1:250 dilution of a previous lot was used on immunohistochemistry. Immunocytochemistry: 1:50 – 1:100 dilution of a previous lot was used on immunocytochemistry. Immunoprecipitation: 1:50 dilution of a previous lot was used on immunoprecipitation. Flow Cytometry: 1:20 dilution of a previous lot was used on flow cytometry.

生物信息
免疫原品种	KLH conjugated linear peptide.
表位	C-terminus
宿主	Rabbit
特异性	This antibody recognizes eEf2 at the C-Terminus.
品种反应性	RatHumanMouse
Species Reactivity Note	Proven to react with human, mouse and rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001952
Entrez基因汇总	This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
基因符号	EF2 EEF-2 EF-2
纯化方法	Unpurified
UniProt编号	P13639
UniProt汇总	FUNCTION: This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. LOCATION: Cytoplasm. PTM: Phosphorylation by EF-2 kinase completely inactivates EF-2. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A. SIMILARITY: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

产品使用声明
质量保证	Routinely evaluated by Western Blot on HEK293 cell lysate. Western Blot Analysis: A 1:50,000 dilution of this lot was to detect eEF2 on HEK293 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.