- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Mk, H, R, M, Po, B, Op ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1364 描述 Anti-ECT2 Antibody Alternate Names - protein ECT2
- epithelial cell transforming sequence 2 oncogene
- Epithelial cell-transforming sequence 2 oncogene
背景信息 Epithelial cell-transforming sequence 2 oncogene (ECT2) is a guanine nucleotide exchange factor or GEF that belongs to the Dbl family of Rho GEFs. ECT2 is composed of 2 BRCT domains within the N-terminus, a DBL-homology domain, and a PH domain. Studies have shown ECT2 to localize to both the cell cortex and central spindles, and that it is a crucial part of cleavage furrow formation during cytokenises. Overexpression of cleaved ECT2, containing only the N-terminal domain, has been shown to cause multinucleation. As a GEF it has been shown to bind specifically to Rac proteins, RhoA, and RhoC. 产品信息 格式 Affinity Purified 控制 - A549 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ECT2 Antibody is an antibody against ECT2 for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected ECT2 in A549 cell lysate.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory on Heya8 cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human ECT2 at the C-terminus. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes ECT2 at the
C-terminus.品种反应性 MonkeyHumanRatMousePigBovineOpossum Species Reactivity Note Demonstrated to react with human. Predicted to react with rat, mouse, porcine, bovine, opossum, monkey, and rabbit based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - Q9H8V3
基因符号 - MGC138291
- ARHGEF31
- FLJ10461
纯化方法 Affinity purified UniProt编号 - Q9H8V3
UniProt汇总 FUNCTION: Binds highly specifically to RhoA, RhoC and Rac proteins, but does not appear to catalyze guanine nucleotide exchange By similarity.
SUBUNIT STRUCTURE: Associates with RACGAP1 at anaphase and during cytokinesis.
SEQUENCE SIMILARITES: Contains 2 BRCT domains.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
CAUTION: It is uncertain whether Met-1 or Met-146 is the initiator.产品使用声明 质量保证 Evaluated by Western Blot in A549 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected ECT2 on 10 µg of A549 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,70933,pTriEx™-4 Neo DNA - Novagen
下一件merck millipore产品：merck millipore,默克密理博,AP112P,Goat Anti-Human IgG Antibody, HRP conjugate
相关产品
merck millipore,默克密理博,EZMIFNG,Mouse IFNγ ELISA Kit, EZMIFNG
merck millipore,默克密理博,AB3071,Anti-Aquaporin 0 Antibody
merck millipore,默克密理博,SF-1995,DAXX; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,70933,pTriEx™-4 Neo DNA - Novagen
merck millipore,默克密理博,AP112P,Goat Anti-Human IgG Antibody, HRP conjugate
merck millipore,默克密理博,475988,mTOR Inhibitor III, PP242 - Calbiochem
merck millipore,默克密理博,OP95,Anti-BRCA2 (Ab-1) Mouse mAb (2B)
merck millipore,默克密理博,AB3532P,Anti-Epithelial Sodium Channel-β Antibody