merck millipore,默克密理博,06-991,Anti-PKCε Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,06-991,Anti-PKCε Antibody
产品名称：Anti-PKCε Antibody
产品型号：06-991
Detect PKCε using this Anti-PKCε Antibody validated for use in IH, IP & WB.

merck millipore,默克密理博,06-991,Anti-PKCε Antibody

产品介绍

merck millipore,默克密理博,06-991,Anti-PKCε Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, IHC	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	06-991
品牌系列	Upstate
商名	Upstate
描述	Anti-PKCε Antibody
Alternate Names	protein kinase C, epsilon protein kinase C
背景信息	Members of the protein kinase C family fall into three categories: conventional (PKC alpha, beta I, beta II, gamma), novel (delta, epsilon, eta, mu, theta), and atypical (lambda, zeta). Conventional PKC isoforms are dependent on calcium and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are calcium dependent, and atypical PKC isoforms are calcium and DAG independent.

产品信息
格式	Purified
控制	Jurkat cell lysate. Included Positive Antigen Control: Catalog # 12-303, Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示	Purified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Store at -20°C.

应用
应用	Detect PKCε using this Anti-PKCε Antibody validated for use in IH, IP & WB.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunoprecipitation: An independent lab has reported this antibody immunoprecipitates PKCε. Immunohistochemistry: An independent lab has reported this antibody detects PKCε.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to the C-terminal variable (V5) region, amino acids 726-737 (KGFSYFGEDLMP), of mouse Protein Kinase C Epsilon (PKCε). The immunizing sequence is identical in human, mouse, rat and rabbit.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes PKCε, Mr 95 kDa.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Mouse, human, and rat. Predicted to cross-react with rabbit.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_005400
Entrez基因汇总	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
基因符号	nPKC-epsilon PRKCE MGC125656 PKCE MGC125657
纯化方法	Protein A purfied
UniProt编号	Q02156
UniProt汇总	FUNCTION: SwissProt: Q02156 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. SIZE: 737 amino acids; 83674 Da SUBUNIT: Forms a ternary complex with TRIM63 and GN2BL1 (By similarity). DOMAIN: SwissProt: Q02156 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. PTM: Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on Jurkat cell lysate. Western Blot Analysis: 0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.