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品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB, ICC Rb Purified Polyclonal Antibody 描述 产品目录编号 06-720 品牌系列 Upstate
商名 - Upstate
描述 Anti-HDAC1 Antibody Alternate Names - reduced potassium dependency, yeast homolog-like 1
- histone deacetylase 1
- reduced potassium dependency
背景信息 Mammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all are related to the yeast transcriptional regulator Rpd3p, are identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing. 产品信息 格式 Purified 控制 - NIH/3T3 cell extract
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 Cell Lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, containing 0.05% sodium azide. Frozen at -20ºC. 应用 应用 Use Anti-HDAC1 Antibody (Rabbit Polyclonal Antibody) validated in EA, ICC, IP, WB to detect HDAC1 also known as histone deacetylase 1. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation:
5 μg of a previous lot immunoprecipitated HDAC1 from 500 μg of 3T3/A31 RIPA lysate.
Immunocytochemistry:
This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.
HDAC Activity:
This antibody has been reported to immunoprecipitate active HDAC.生物信息 免疫原品种 6X-His-tagged fragment representing amino acids 53-482 of mouse histone deacetylase 1 (HDAC1). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes and is specific for HDAC1, Mr 65 kDa. May detects a non-specific protein at ~35 kDa. 同种型 IgG 品种反应性 MouseHumanRat Species Reactivity Note Mouse, rat and human. Other species cross-reactivity not tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004955
Entrez基因汇总 Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq] 基因符号 - RPD3
- HDAC1
- DKFZp686H12203
- HD1
- RPD3L1
- GON-10
纯化方法 Protein A chromatography UniProt编号 - Q13547
UniProt汇总 FUNCTION: SwissProt: Q13547 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
SIZE: 482 amino acids; 55103 Da
SUBUNIT: Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Associates with the 9-1-1 complex; interacts with HUS1. Found in a complex with DNMT3A and HDAC7. Interacts with BCOR, BRMS1L, DAXX, DNMT1, EP300, HCFC1, PCAF, PHB2, MIER1, JMJD2A/JHDM3A, MINT, NRIP1, RERE, SETDB1, SUV39H1, TGIF, TGIF2, UHRF1 and UHRF2. Interacts with the non-histone region of H2AFY. Interacts with HDAC9. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with BANP, CBFA2T3 and JARID1B. Interacts with SAP30L.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
PTM: Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. & Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
SIMILARITY: SwissProt: Q13547 ## Belongs to the histone deacetylase family. Type 1 subfamily.产品使用声明 质量保证 Routinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µg
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下一件merck millipore产品：merck millipore,默克密理博,03-248,RIPAb+™ pan Ago - RIP Validated Antibody and Primer Set
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