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merck millipore,默克密理博,06-1339,Anti-CHD-1 Antibody
产品名称：Anti-CHD-1 Antibody
产品型号：06-1339
Anti-CHD-1 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-1 also known as chromodomain helicase DNA binding protein 1, ATP-dependent helicase CHD1 & has been validated in WB, ICC.
merck millipore,默克密理博,06-1339,Anti-CHD-1 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1339 描述 Anti-CHD-1 Antibody Alternate Names - chromodomain-helicase-DNA-binding protein 1
- ATP-dependent helicase CHD1
- chromodomain helicase DNA binding protein 1
背景信息 Chromodomain-helicase-DNA-binding protein 1 (CHD-1) is a member of the SNF2/RAD54 helicase family and is comprised of two chromo domains, one helicase ATP-binding domain, and one helicase C-terminal domain. CHD-1 is a regulator for RNA polymerase I transcription, polymerase II transcription, and a negative regulator for DNA replication. It is a chromatin-remodeling factor dependent on ATP and acting as a component for substrate recognition in HAT complex SAGA transcription. Studies have demonstrated CHD-1 involvement in HDAC activity and in the regulation of pre-mRNA splicing efficacy. CHD-1 is a required component in the maintenance of embryonic stem (ES) cell pluripotency. 产品信息 格式 Affinity Purified 控制 - HeLa nuclear extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-CHD-1 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-1 also known as chromodomain helicase DNA binding protein 1, ATP-dependent helicase CHD1 & has been validated in WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected CHD-1 in NIH/3T3 and HeLa cells. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human CHD-1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human and mouse.
Other homologies: Rat (91% sequence homology). Chimpanzee (99% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001261.2
Entrez基因汇总 The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq]. 基因符号 - CHD-1
- CHD1
纯化方法 Affinity Purified UniProt编号 - O14646
UniProt汇总 FUNCTION: ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells. Ref.10
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.
SUBUNIT STRUCTURE: Component of the SAGA complex (By similarity). Interacts with BCLAF1, NCoR, SRP20 and SAFB By similarity. Specifically interacts with methylated H3K4me2 and H3K4me3. Interacts with the FACT complex, the PAF complex and the U2 snRNP. Interacts directly with PAF1, SFA3A1, SFA3A2, SFA3A3, SNF2 and SSRP1.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity). Note: Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis (By similarity).
DOMAIN: The 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3). Ref.8
SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.25 µg/mL of this antibody detected CHD-1 in 10 µg of HeLa nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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