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品种反应性 主要应用 宿主 格式 抗体类型 Rhesus Macaque, H, Eq IHC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 06-1110 Replaces 04-451 描述 Anti-TRAF6 Antibody Alternate Names - TNF receptor-associated factor 6
- Interleukin-1 signal transducer
- RING finger protein 85
背景信息 TNF receptor-associated factor 6 is a member of the TNF receptor associated factor (TRAF) protein family. TNF receptor-associated factor 6 proteins play an important role as adaptor protein for cell survival, proliferation, differentiation and stress responses. TNF receptor-associated factor 6 has unique binding behavior that result in signaling responses from the interleukin-1 receptor (IL-1R) toll-like receptor. TNF receptor-associated factor 6 mediates signaling indirectly with IL-1R/TLR through IRAK. This leads to activation of NF-κB and MAP kinase signaling pathways though downstream association with the TAB/TAK-1 complex. TNF receptor-associated factor 6 is also involved in the activation of Akt/PKB and serves as a direct E3 ligase which is essential for Akt ubiquitination. 产品信息 格式 Purified 控制 - Jurkat cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Detect TRAF6 using this Anti-TRAF6 Antibody validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected TRAF6 on 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:300 dilution from a previous lot detected TRAF6 in ductal carcinoma tissue.
Optimal Staining With Citrate pH 6.0 Epitope Retrieval: Human TMA.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the MATH (meprin-associated Traf homology) domain of human TRAF6. 表位 MATH (meprin-associated Traf homology) Domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the MATH (meprin-associated Traf homology) domain of TRAF6. 品种反应性 Rhesus MacaqueHumanHorse Species Reactivity Note Demonstrated to react with human.
Predicted to react with equine and rhesus monkey based on 100% sequence homology.
Mouse, rat, and ox ( 93% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004611
Entrez基因汇总 The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported. [provided by RefSeq]. 基因符号 - RNF85
- TRAF6
纯化方法 Protein A purfied UniProt编号 - Q9Y4K3
UniProt汇总 FUNCTION: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Also involved in the IL-1 signaling pathway via MYD88 and IRAK kinases. Seems to be involved in IL-17 signaling (By similarity). Mediates activation of NF-kappa-B and JNK. May function as an E3 ubiquitin ligase.
SIZE: 522 amino acids; 59573 Da
SUBUNIT: Homotrimer (Probable). Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK1, IRAK2, IRAK3, IRAK4, RIPK2, MAP3K1, MAP3K5, MAP3K14, CSK, and TRAF- interacting protein TRIP and TRAF and TNF receptor associated protein TTRAP. Interacts with IL17R. Interacts with SQSTM1 bridging NTRK1 and NGFR. Forms a ternary complex with SQSTM1 and PRKCZ (By similarity). Interacts with PELI1, PELI2 and PELI3. Binds UBE2V1. Interacts with MAVS/IPS1. Interacts with TAX1BP1. Interacts with IL1RL1. Interacts with TRAFD1. Interacts with ZNF675.
SUBCELLULAR LOCATION: Cytoplasm (Probable).TISSUE SPECIFICITY: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
DOMAIN: The coiled coil domain mediates homo- and hetero- oligomerization. & The MATH/TRAF domain binds to receptor cytoplasmic domains.
PTM: Polyubiquitinated (By similarity).
SIMILARITY: Contains 1 MATH domain. & Contains 1 RING-type zinc finger. & Contains 2 TRAF-type zinc fingers.产品使用声明 质量保证 Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected TRAF6 in 10 µg of Jurkat cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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