- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, Ca, M ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1002 描述 Anti-LAP2 Antibody Alternate Names - lamina-associated polypeptide 2
- LEM domain containing 4
- TP alpha
- TP beta/gamma
- TPRP isoform alpha
- TPRP isoforms beta/gamma
- Thymopoietin isoform alpha
- Thymopoietin, isoforms beta/gamma
- Thymopoietin-related peptide isoform alpha
- Thymopoietin-related peptide isoforms beta/gamma
- Thymopoietin-related peptide isoforms beta/gamma
背景信息 The inner membrane of the nuclear envelope is connected to the nuclear lamina. The main components of the nuclear lamina are Type V intermediate filaments called lamins and lamin binding proteins including lamin B receptor, emerin, MANI, LAP1 (3 isoforms), and LAP2 (several isoforms). The lamins are divided into two groups: the constitutively expressed lamin B and the developmentaly regulated lamin A.
The best characterized isoforms of lamin binding protein LAP2 are LAP2α and LAP2β. LAP2β is a type II membrane protein in the inner nuclear membrane that binds lamin B and is important to cell viability and controlling nuclear lamina growth. LAP2α has been characterized as a nucleoplasmic protein that interacts with A-type lamins to control gene expression, transcription, and chromatin organization.
产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl, with 0.05% sodium azide. 应用 应用 Anti-LAP2 Antibody is an antibody against LAP2 for use in WB, IC & IF. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunofluorescence:
Representative lot data. This antibody was used to detect the nuclear lamina by immunofluorescence.
Immunocytochemistry:
Representative lot data.
A previous lot was used in confocal fluorescent analysis of A431, HeLa and NIH/3T3 cells using anti-LAP2 rabbit polyclonal antibody (Red).
Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.
生物信息 免疫原品种 KLH-conjugated synthetic linear peptide corresponding to LAP2. 宿主 Rabbit 特异性 This antibody recognizes LAP2. 品种反应性 RatHumanCanineMouse Species Reactivity Note Tested on human and mouse. Predicted to cross-react with canine (dog) and rat based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003276
基因符号 - thymopoietin
- CMD1T
- LAP2
- LEMD4
- MGC61508
- PRO0868
- TP
纯化方法 Affinity Purfied UniProt编号 - P42167
UniProt汇总 FUNCTION: May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95.
TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.
SUBUNIT STRUCTURE: Interacts with LMNB1, LMNB2, BANF1, NAKAP95, GMCL and chromosomes By similarity.
SUBCELLULAR lOCATION: Nucleus inner membrane; Single-pass type II membrane protein. Note= Tightly associated with the nuclear lamina.
TISSUE SPECIFICTY: Expressed in many tissues. Most abundant in adult thymus and fetal liver.
DOMAIN: Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1.
PTM: Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes By similarity.
PHARMACEUTICAL USE: TP5 is available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Used in primary and secondary immune deficiencies, autoimmunity, infections and cancer.
SIMILARITY: Belongs to the LEM family.
Contains 1 LEM domain.
Contains 1 LEM-like domain.
产品使用声明 质量保证 Evaluated by western blot on HeLa cell lysate.
Western Blot Analysis:
A 1:1000-1:3000 dilution of this antibody was used to detect LAP2 in HeLa cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,12-586,TK Calibrator 2
下一件merck millipore产品：merck millipore,默克密理博,09-809,Anti-JMJD2A Antibody
相关产品
merck millipore,默克密理博,SF-1334,BIRC3; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,517312,Anti-P-Glycoprotein Mouse mAb (C494)
merck millipore,默克密理博,12-586,TK Calibrator 2
merck millipore,默克密理博,09-809,Anti-JMJD2A Antibody
merck millipore,默克密理博,SF-1328,BCL6; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,SKBM04760,Strat-M Membrane, Transdermal Diffusion Test Model, 47mm
merck millipore,默克密理博,AP113A-K,Goat anti-Human IgG, Fc, Alkaline Phosphatase conjugate (1mg)
merck millipore,默克密理博,04-1550,Anti-SC-35 Antibody, clone 1SC-4F11