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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-844 品牌系列 Upstate
商名 - Upstate
描述 Anti-PLK1 Antibody, clone 35-206 Alternate Names - polo-like kinase 1 (Drosophila)
- Polo-like kinase 1
- Serine/threonine-protein kinase 13
- cell cycle regulated protein kinase
- polo (Drosophia)-like kinase
- polo like kinase
- polo-like kinase (Drosophila)
背景信息 Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. 产品信息 格式 Purified 控制 - RIPA lysates from 3T3/A31 cells
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Protein A purified mouse IgG2b in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C. 应用 应用 Anti-PLK1 Antibody, clone 35-206 detects level of PLK1 & has been published & validated for use in WB, IP, IC, IH. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation: An independent laboratory has reported that a previous lot of this antibody can be used to immunoprecipitate PLK1.
Immunohisto/immunocytochemistry: An independent laboratory reported that 1 μg/mL of a previous lot of this antibody showed positive immunostaining for PLK1 in paraformaldehyde treated, Triton X-100 fixed cells.生物信息 免疫原品种 6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK. 克隆 clone 35-206 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 PLK1 同种型 IgG2b 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005021
基因符号 - STPK13
- PLK1
- PLK
- PLK-1
纯化方法 Protein A purfied UniProt编号 - P53350
UniProt汇总 FUNCTION: SwissProt: P53350 # May be required for cell division and may have a role during G1 or S phase.
SIZE: 603 amino acids; 68255 Da
SUBUNIT: Interacts with CEP170 and EVI5.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Placenta and colon.
DEVELOPMENTAL STAGE: Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137. & Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.
SIMILARITY: SwissProt: P53350 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. & Contains 2 POLO box domains. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by western blot on RIPA lysates from 3T3/A31 cells.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected PLK1 in RIPA lysates from 3T3/A31 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at from date of receipt. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB2146Z,Anti-ICAM-1 Antibody, clone P2A4, Azide Free
下一件merck millipore产品：merck millipore,默克密理博,3098,Nuclease-free Water
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