merck millipore,默克密理愽
  • merck millipore,默克密理博,05-802,Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

  • merck millipore,默克密理博,05-802,Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

    产品名称：Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal
    产品型号：05-802
    Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50 is an antibody against phospho-Akt1/PKBα (Thr308) for use in WB.

    merck millipore,默克密理博,05-802,Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

  • 产品介绍
  • merck millipore,默克密理博,05-802,Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, HWBRbPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-802
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal
    背景信息 Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.
    产品信息
    格式 Purified
    控制
    • PDGF treated 3T3/NIH cells
    演示 Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
    应用
    应用 Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50 is an antibody against phospho-Akt1/PKBα (Thr308) for use in WB.
    主要应用
    • Western Blotting
    生物信息
    免疫原品种 peptide containing the sequence MK[pT]FC in which pT corresponds to phospho-threonine at residue 308 of mouse Akt
    克隆 NL50
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes phosphorylated Akt1/PKBaα.
    同种型 IgG
    品种反应性 MouseHuman
    Species Reactivity Note Predicted to cross-react with rat, bovine and chicken based on sequence homology
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_005163
    Entrez基因汇总 The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
    基因符号
    • AKT
    • AKT1
    • RAC
    • PRKBA
    • RAC-ALPHA
    • PKB
    • MGC99656
    • RAC-PK-alpha
    • C-AKT
    修改
    • Phosphorylation
    纯化方法 Protein A purfied
    UniProt编号
    • P31749
    UniProt汇总 FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
    SIZE: 480 amino acids; 55686 Da
    SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
    SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
    TISSUE SPECIFICITY: In all human cell types so far analyzed.
    DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
    PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
    SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
    产品使用声明
    质量保证 routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells stimulated with 100ng/ml PDGF for 20 minutes
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,05-802,Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

上一件merck millipore产品：merck millipore,默克密理博,12-451,Autocamtide 2, biotin conjugate
下一件merck millipore产品：merck millipore,默克密理博,FCCS025550,FlowCellect™ Multi-STAT Activation Profiling Kit


相关产品

merck millipore,默克密理博,ABE210,Anti-TRF1 Antibody merck millipore,默克密理博,ABE210,Anti-TRF1 Antibody

merck millipore,默克密理博,04-805,Anti-SET7/9 Antibody, clone 5F2.3 merck millipore,默克密理博,04-805,Anti-SET7/9 Antibody, clone 5F2.3

merck millipore,默克密理博,422426,KGF/FGF-7, Mouse, Recombinant merck millipore,默克密理博,422426,KGF/FGF-7, Mouse, Recombinant

merck millipore,默克密理博,PF017,TGF-β2, Human, Recombinant, E. coli merck millipore,默克密理博,PF017,TGF-β2, Human, Recombinant, E. coli

merck millipore,默克密理博,12-451,Autocamtide 2, biotin conjugate merck millipore,默克密理博,12-451,Autocamtide 2, biotin conjugate

merck millipore,默克密理博,FCCS025550,FlowCellect™ Multi-STAT Activation Profiling Kit merck millipore,默克密理博,FCCS025550,FlowCellect™ Multi-STAT Activation Profiling Kit

merck millipore,默克密理博,CC1059,MMP-7, human, recombinant active form merck millipore,默克密理博,CC1059,MMP-7, human, recombinant active form

merck millipore,默克密理博,108411,苏氨酸 merck millipore,默克密理博,108411,苏氨酸

merck millipore,默克密理博,ABF11,Anti-C4d Antibody merck millipore,默克密理博,ABF11,Anti-C4d Antibody

merck millipore,默克密理博,ECM104,Millicoat™ Human Collagen Type I Coated Strips (96-Wells) merck millipore,默克密理博,ECM104,Millicoat™ Human Collagen Type I Coated Strips (96-Wells)


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部