merck millipore,默克密理博,05-791R,Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

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merck millipore,默克密理博,05-791R,Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal
产品名称：Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal
产品型号：05-791R
Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304 is a rabbit monoclonal antibody for detection of mono/di/trimethyl-Histone H3 (Lys4) also known as histone H3.1, H3 histone family member More>>

merck millipore,默克密理博,05-791R,Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

产品介绍

merck millipore,默克密理博,05-791R,Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	05-791R
Replaces	05-791
描述	Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal
Alternate Names	H3 histone family, member I histone H3.1 histone H3/a histone H3/b histone H3/c histone H3/d histone H3/f histone H3/h histone H3/I histone H3/j histone H3/k histone H3/l histone 1, H3f OTTHUMP00000016151
背景信息	Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone methylation is primarily associated with heterochromatin and gene silencing.

产品信息
格式	Purified
控制	Recombinant Histone H3 and HeLa acid extract.
演示	Purified rabbit monoclonal IgG containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, 40% Glycerol and 0.05% sodium azide.

应用
应用	Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304 is a rabbit monoclonal antibody for detection of mono/di/trimethyl-Histone H3 (Lys4) also known as histone H3.1, H3 histone family member I & has been validated in WB, PIA.
主要应用	Western Blotting
应用说明	Peptide Block Analysis: Peptides corresponding to Histone H3 mono, di, or trimethylated at Lys4 specifically blocked binding of anti-mono/di/trimethyl-Histone H3 (Lys4) in HeLa acid extracts.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human Histone H3 mono/di/tri-methylated at Lys4.
表位	Mono/di/tri-methylated Lys4.
克隆	AW304
宿主	Rabbit
特异性	This antibody recognizes Histone H3 mono/di/tri-methylated at Lys4.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Demonstrated to react with Human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_066298
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
基因符号	H3FJ HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J
修改	Methylation
纯化方法	Protein A
UniProt编号	P68431
UniProt汇总	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCALIZATION: Nucleus. Chromosome.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: 1:8,000 dilution of this antibody detected Histone H3 in 10 µg of HeLa acid extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.