merck millipore,默克密理博,05-778,Anti-Rho (-A Antibody, -B, -C), clone 55

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merck millipore,默克密理博,05-778,Anti-Rho (-A Antibody, -B, -C), clone 55
产品名称：Anti-Rho (-A Antibody, -B, -C), clone 55
产品型号：05-778
Detect Rho (-A, -B, -C) using this Anti-Rho (-A Antibody, -B, -C), clone 55 validated for use in IC & WB.

merck millipore,默克密理博,05-778,Anti-Rho (-A Antibody, -B, -C), clone 55

产品介绍

merck millipore,默克密理博,05-778,Anti-Rho (-A Antibody, -B, -C), clone 55

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, ICC	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-778
品牌系列	Upstate
商名	Upstate
描述	Anti-Rho (-A Antibody, -B, -C), clone 55
Alternate Names	small GTP binding protein RhoA Aplysia ras-related homolog 12 oncogene RHO H12 ras homolog gene family, member A member A
背景信息	Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganization, and membrane trafficking.

产品信息
格式	Purified
控制	HL-60 cell lysate.
演示	Purified mouse monoclonal IgG1 in buffer containing BSA containing 0.09% sodium azide and 50% glycerol.

应用
应用	Detect Rho (-A, -B, -C) using this Anti-Rho (-A Antibody, -B, -C), clone 55 validated for use in IC & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry: This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence.

生物信息
免疫原品种	Recombinant human RhoA containing amino acids 1-155. Clone 55.
克隆	55
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes Rho (-A, -B, -C), Mr 22 kDa.
同种型	IgG1
品种反应性	RatHumanMouse
Species Reactivity Note	Human, rat and mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001655
Entrez基因汇总	This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
基因符号	H12 RHOA ARHA ARH12 RHO12 RHOH12 Rho12 RhoA
纯化方法	Protein G purfied
UniProt编号	P61586
UniProt汇总	FUNCTION: SwissProt: P62745 # Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. SIZE: 196 amino acids; 22123 Da SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13. SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. PTM: Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. SIMILARITY: SwissProt: P62745 ## Belongs to the small GTPase superfamily. Rho family. MISCELLANEOUS: RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.

产品使用声明
质量保证	Routinely evaluated by western blot on RIPA lysates from Jurkat or HL-60 cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.