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merck millipore,默克密理博,05-777,Anti-Phosphotyrosine Antibody, recombinant clone 4G10
产品名称：Anti-Phosphotyrosine Antibody, recombinant clone 4G10
产品型号：05-777
Anti-Phosphotyrosine Antibody, clone 4G10 is a recombinant antibody that detects tyrosine phosphorylated proteins in all species. This unique recombinant antibody is validated for use in IC, IH, IP, More>>
merck millipore,默克密理博,05-777,Anti-Phosphotyrosine Antibody, recombinant clone 4G10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Vrt WB M Purified Monoclonal Antibody 描述 产品目录编号 05-777 品牌系列 Upstate
商名 - Upstate
描述 Anti-Phosphotyrosine Antibody, recombinant clone 4G10® 背景信息 Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments. 产品信息 格式 Purified 控制 - Positive Control Included: EGF stim A431 Cell Lysate (12-302).
演示 Protein G purified recombinant 4G10 mouse IgG2bk in 0.01M Phosphate Buffer containing no preservative, pH 7.1 应用 应用 Anti-Phosphotyrosine Antibody, clone 4G10 is a recombinant antibody that detects tyrosine phosphorylated proteins in all species. This unique recombinant antibody is validated for use in IC, IH, IP, WB and is published in peer review journals. 主要应用 - Western Blotting
生物信息 免疫原品种 Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via Nickel affinity matrices. 克隆 4G10® 宿主 Mouse 特异性 Recognizes tyrosine-phosphorylated proteins from all species. 同种型 IgG2bκ 品种反应性 Vertebrates 抗体类型 Monoclonal Antibody 修改 - Phosphorylation
纯化方法 Protein G purfied 产品使用声明 质量保证 routinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 1 mg
上一件merck millipore产品：merck millipore,默克密理博,UFC810008,Amicon Ultra-4，PLHK Ultracel-PL 超滤膜，100 kDa
下一件merck millipore产品：merck millipore,默克密理博,118410,Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt
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