merck millipore,默克密理愽
  • merck millipore,默克密理博,04-1572-I,Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

  • merck millipore,默克密理博,04-1572-I,Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

    产品名称：Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
    产品型号：04-1572-I
    Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.

    merck millipore,默克密理博,04-1572-I,Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

  • 产品介绍
  • merck millipore,默克密理博,04-1572-I,Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    MELISA, ChIP, WBRCulture SupernatantMonoclonal Antibody
    描述
    产品目录编号 04-1572-I
    Replaces 04-1572
    描述 Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
    Alternate Names
    • RNA-directed RNA polymerase II subunit RPB1
    • DNA-directed RNA polymerase II subunit RPB1
    • RNA polymerase II subunit B1
    • DNA-directed RNA polymerase II subunit A
    • DNA-directed RNA polymerase III largest subunit
    背景信息 RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at 'Ser-7' of the heptapepdtide repeat. This antibody recognizes the 'Ser-5' CTD residue of RPB1.
    产品信息
    格式 Culture Supernatant
    控制
    • Untreated and lambda phosphotase treated NIH/3T3 cell lysates
    演示 Rat monoclonal IgG2aκ with 0.05% sodium azide.
    应用
    应用 Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.
    主要应用
    • ELISA
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    应用说明 Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).

    ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
    生物信息
    免疫原品种 Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminal domain (CTD).
    表位 Phosphorylated Ser5 at the C-terminus domain (CTD).
    克隆 3E8
    宿主 Rat
    特异性 This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminus domain (CTD).
    同种型 IgG2aκ
    品种反应性 Mouse
    Species Reactivity Note Demonstrated to react with Mouse. Predicted to react with Human based upon immunogen design.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_000928
    基因符号
    • POLR2
    • POLR2A
    纯化方法 Unpurified
    UniProt编号
    • P24928
    产品使用声明
    质量保证 Evaluated by Western Blot in untreated and lambda phosphotase treated NIH/3T3 cell lysates.

    Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.
    Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,04-1572-I,Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

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下一件merck millipore产品：merck millipore,默克密理博,03-110,RIPAb+ Ago2 - RIP Validated Antibody and Primer Set


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