merck millipore,默克密理博,05-716,Anti-Myosin Heavy Chain Antibody, clone A4.1025

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merck millipore,默克密理博,05-716,Anti-Myosin Heavy Chain Antibody, clone A4.1025
产品名称：Anti-Myosin Heavy Chain Antibody, clone A4.1025
产品型号：05-716
Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.

merck millipore,默克密理博,05-716,Anti-Myosin Heavy Chain Antibody, clone A4.1025

产品介绍

merck millipore,默克密理博,05-716,Anti-Myosin Heavy Chain Antibody, clone A4.1025

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Rb, H, R, M, Zebrafish, Dr	WB, IHC	M	Ascites	Monoclonal Antibody

描述
产品目录编号	05-716
品牌系列	Upstate
商名	Upstate
描述	Anti-Myosin Heavy Chain Antibody, clone A4.1025
Alternate Names	myosin, heavy polypeptide 1, skeletal muscle, adult Myosin heavy chain 1 Myosin heavy chain 2x Myosin heavy chain IIx/d Myosin heavy chain, skeletal muscle, adult 1 myosin, heavy chain 1, skeletal muscle, adult adult 1 myosin heavy chain 1 adult
背景信息	Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.

产品信息
格式	Ascites
控制	RIPA lysates from C2C12 cells
演示	Unpurified mouse ascites monoclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

应用
应用	Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunohistochemistry: This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).

生物信息
免疫原品种	Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.
克隆	A4.1025
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.
同种型	IgG
品种反应性	RabbitHumanRatMouseZebrafishDrosophila
Species Reactivity Note	Human, mouse, rat, rabbit, zebrafish and Drosophila.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_005963
Entrez基因汇总	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
基因符号	MYHSA1 MYH1 MyHC-2X/D MGC133384 MYHa MyHC-IIx/d MyHC-2x Myosin-1
纯化方法	Unpurified
UniProt编号	P12882
UniProt汇总	FUNCTION: SwissProt: P12882 # Muscle contraction. SIZE: 1939 amino acids; 223115 Da SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. DOMAIN: SwissProt: P12882 The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. SIMILARITY: Contains 1 IQ domain. & Contains 1 myosin head-like domain. MISCELLANEOUS: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

产品使用声明
质量保证	Routinely evaluated by Western Blot on RIPA lysates from C2C12 cells. Western Blot Analysis: 1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.