- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-504 品牌系列 Upstate
商名 - Upstate
描述 Anti-IQGAP1 Antibody, clone AF4 产品信息 格式 Purified 演示 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% 应用 应用 Detect IQGAP1 using this Anti-IQGAP1 Antibody, clone AF4 validated for use in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 A Glu-Glu tagged C-terminal fragment corresponding to amino acids 863-1657 of human IQGAP1, purified from baculovirus infected insect cells 克隆 clone AF4 宿主 Mouse 特异性 IQGAP1, not IQGAP2 同种型 IgG 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003870
Entrez基因汇总 This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. 基因符号 - p195
- IQGAP1
- HUMORFA01
- SAR1
- KIAA0051
纯化方法 Protein G chromatography UniProt编号 - P46940
UniProt汇总 FUNCTION: SwissProt: P46940 # Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
SIZE: 1657 amino acids; 189252 Da
SUBUNIT: Interacts with CDC42, both in its GTP-bound state and in its nucleotide-free state. Also interacts with RAC1, but not with RHOA.
SUBCELLULAR LOCATION: Cell membrane.
TISSUE SPECIFICITY: Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
DOMAIN: SwissProt: P46940 Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
PTM: Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser- 1443 phosphorylation enhances the ability to promote neurite outgrowth.
SIMILARITY: Contains 1 CH (calponin-homology) domain. & Contains 4 IQ domains. & Contains 1 Ras-GAP domain. & Contains 1 WW domain.产品使用声明 质量保证 routinely evaluated by immunoblot on HepG2 RIPA cell lysate 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,1381,ε-Amino-n-caproic Acid - CAS 60-32-2 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,AB1934,Anti-Integrin α1 Subunit Antibody, cytoplasmic/intracellular
相关产品
merck millipore,默克密理博,475857,Mirk/Dyrk1B Inhibitor, Compound A - Calbiochem
merck millipore,默克密理博,227040,Anti-Cholera Toxin, B-Subunit Goat pAb
merck millipore,默克密理博,OP188,Anti-FAP Mouse mAb (F11-24)
merck millipore,默克密理博,MAB8510,Anti-Human Metapneumovirus 75.1 Antibody, clone 1B7
merck millipore,默克密理博,1381,ε-Amino-n-caproic Acid - CAS 60-32-2 - Calbiochem
merck millipore,默克密理博,AB1934,Anti-Integrin α1 Subunit Antibody, cytoplasmic/intracellular
merck millipore,默克密理博,AB6016,Anti-F-actin-capping protein subunit alpha-1 Antibody
merck millipore,默克密理博,ABN309,Anti-SAPAP1 Antibody
merck millipore,默克密理博,MAB10401,Anti-Plexin-D1 Antibody, clone 19C6.1