merck millipore,默克密理愽
  • merck millipore,默克密理博,05-483,Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2

  • merck millipore,默克密理博,05-483,Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2

    产品名称：Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2
    产品型号：05-483
    Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2 detects level of phospho-EGFR (Tyr1173) & has been published & validated for use in ELISA, IC, IP & WB.

    merck millipore,默克密理博,05-483,Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2

  • 产品介绍
  • merck millipore,默克密理博,05-483,Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Ca, M, R, HWB, ELISA, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-483
    Replaces 04-341
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2
    Alternate Names
    • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
    • Receptor tyrosine-protein kinase ErbB-1
    • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
    • cell growth inhibiting protein 40
    • cell proliferation-inducing protein 61
    • epidermal growth factor receptor
    • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
    • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog
    背景信息 The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
    EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers.
    EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues in the C-terminal domain of EGFR occurs. These are Y992, Y1045, Y1068, Y1148 and Y1173. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding SH2 domains. These downstream signaling proteins initiate several signal transduction cascades, principally the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. Such proteins modulate phenotypes such as cell migration, adhesion, and proliferation. The kinase domain of EGFR can also cross-phosphorylate tyrosine residues of other receptors it is aggregated with, and can itself be activated in that manner.
    产品信息
    格式 Purified
    控制
    • EGF stimulated HeLa cells, EGF stimulated A431 cells.
    演示 Purified mouse monoclonal IgG1κ in buffer containing 1 mL of PBS, 0.09% sodium azide, PEG, and sucrose.

    Rehydration:
    Rehydrate with 100 μL of sterile, distilled water to make a 1 mg/mL solution.
    应用
    应用 Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2 detects level of phospho-EGFR (Tyr1173) & has been published & validated for use in ELISA, IC, IP & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    应用说明 Immunoprecipitation:
    1-10 μg of a previous lot of this antibody is reported by an independent lab to immunoprecipitate phosphorylated EGFR from 106 vanadate treated A431 cells.

    ELISA:
    0.05 μg/mL of a previous lot of this antibody is recommended by an independent lab for ELISA testing.

    Immunocytochemistry:
    1-10 μg/mL of a previous lot of this antibody is recommended by an independent lab for immunocytochemistry testing.
    生物信息
    免疫原品种 KLH-conjugated, synthetic peptide containing the Y1173 motif (...NAE[pY1173]LRV...) of the human EGFR.
    克隆 9H2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes the motif NAE[pY]LRV corresponding to the major auto-phosphorylation site of the human EGFR, Mr 175 kDa. Tyr1173 corresponds to Tyr1197 in the human (isoforms a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins. This antibody does not interact with the non-phosphorylated EGFR or with other unrelated phosphotyrosine proteins.
    同种型 IgG1κ
    品种反应性 CanineMouseRatHuman
    Species Reactivity Note Human, mouse, rat and dog.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_005228
    Entrez基因汇总 The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].

    Transcript Variant: This variant (1) is the longest transcript and it encodes the longest isoform (a).
    基因符号
    • mENA
    • EGFR
    • ERBB1
    • ERBB
    修改
    • Phosphorylation
    纯化方法 Thiophilic adsoption and size exclusion chromatography
    UniProt编号
    • P00533
    UniProt汇总 FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
    SIZE: 1210 amino acids; 134277 Da
    SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
    SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
    TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
    PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
    SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
    MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on untreated A431 and EGF treated A431 lysates.

    Western Blot Analysis:
    1:500 dilution of this lot detected PHOSPHO-EGF RECEPTOR on 10 μg of EGF treated A431 lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Lyophilized:
    stable for 1 year at -20°C from date of shipment.

    Rehydrated:
    Stable for 3 months at 4°C. Aliquot solution to store frozen and avoid repeated freeze thaw cycles.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,05-483,Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2

上一件merck millipore产品：merck millipore,默克密理博,07-1007,Anti-IKKα Antibody
下一件merck millipore产品：merck millipore,默克密理博,71071,pET-41 Ek/LIC Vector Kit - Novagen


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