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品种反应性 主要应用 宿主 格式 抗体类型 R, Av, Ca, H, M ICC, WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 05-481 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-MAP Kinase1/2 Antibody Alternate Names - mitogen-activated protein kinase 3
- Extracellular signal-regulated kinase
- Insulin-stimulated MAP2 kinase
- MAP kinase 1
- MAPK 1
- Microtubule-associated protein 2 kinase
- extracellular signal-related kinase 1
背景信息 The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y. 产品信息 格式 Purified 控制 - Included Positive Control Lysate: Catalog # 12-302, EGF-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg (20 μL) of reduced lysate per lane for minigels.
演示 Purified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose.
Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.应用 应用 Detect phospho-MAP Kinase1/2 using this Anti-phospho-MAP Kinase1/2 validated for use in WB, IP, ELISA & IC. 主要应用 - Immunocytochemistry
- Western Blotting
- ELISA
应用说明 Immunoprecipitation: Use 1-10 μg per immuno-precipitation. 生物信息 免疫原品种 KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4 克隆 clone 12D4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Specific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2. 同种型 IgG1κ 品种反应性 RatAvianCanineHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002745, NM_002746
Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. ... 基因符号 - ERK-1
- MAPK3
- MGC20180
- ERT2
- HUMKER1A
- p44-MAPK
- PRKM3
- ERK1
- p44erk1
- p44mapk
- P44MAPK
- HS44KDAP
- p44-ERK1
- P44ERK1
修改 - Phosphorylation
纯化方法 Thiophilic adsoption and size exclusion chromatography UniProt编号 - P27361
UniProt汇总 FUNCTION: SwissProt: P27361 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 379 amino acids; 43136 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P27361 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ...产品使用声明 质量保证 Evaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells.
Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at from date of receipt. 包装信息 数量 100 µg
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