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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, H, R, M, B WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-480 品牌系列 Upstate
商名 - Upstate
描述 Anti-Eck/EphA2 Antibody, clone D7 Alternate Names - receptor protein tyrosine kinase regulated by p53 and E2F-1
- EPH receptor A2
- Epithelial cell kinase
- Tyrosine-protein kinase receptor ECK
- ephrin receptor EphA2
- epithelial cell receptor protein tyrosine kinase
- protein tyrosine kinase
背景信息 EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.产品信息 格式 Purified 控制 - A431 cell lysate, CHO cell lysate, foreskin fibroblasts, 3T3/A31 cells, L6 cells.
Included Positive Antigen Control: Catalog # 12-301, non stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示 Purified mouse monoclonal IgG1 in buffer containing 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol. 应用 应用 This Anti-Eck/EphA2 Antibody, clone D7 is validated for use in IC, IP, EA, WB for the detection of Eck/EphA2. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Protein Kinase Assay:
A previous lot of this antibody has been reported to have been used in an immunoprecipitation autophosphorylation assay, using a Mn-PIPES reaction buffer (Romer, L., 1994).
Immunoprecipitation:
A previous lot of this antibody has been reported to immunoprecipitate Eck from 500 µg of a human breast epithelial cell line which had been lysed in TBS containing 1% Triton X-100. Use 1-4 µg per reaction.
Immunocytochemistry:
A previous lot of this antibody has been reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7% formaldehyde solution and permeabilized with 0.5% Triton X-100 in TBS.生物信息 免疫原品种 Native protein isolated by purification of phosphotyrosine-containing proteins. Clone D7. 克隆 D7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Epithelial Cell Kinase (Eck/EphA2), Mr 140 kDa 同种型 IgG1 品种反应性 CanineHumanRatMouseBovine Species Reactivity Note Human, mouse, rat, bovine and canine. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004431
Entrez基因汇总 This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. 基因符号 - ECK
- EPHA2
- EphA2
纯化方法 Protein G purfied UniProt编号 - P29317
UniProt汇总 FUNCTION: SwissProt: P29317 # Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5.
SIZE: 976 amino acids; 108254 Da
SUBUNIT: Interacts with SLA (By similarity). Interacts with INPPL1/SHIP2.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
SIMILARITY: SwissProt: P29317 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain. & Contains 1 SAM (sterile alpha motif) domain.产品使用声明 质量保证 Routinely evaluated by immunoblot on RIPA lysates from human A431, foreskin fibroblasts, murine 3T3/A31 or rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Eck in RIPA lysates from human A431 and previously from foreskin fibroblasts, murine 3T3/A31 and rat L6 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. NOTE: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.包装信息 数量 200 µg
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