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品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, Ht, M WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-372 品牌系列 Upstate
商名 - Upstate
描述 Anti-GRB2 Antibody, clone 3F2 产品信息 格式 Purified 演示 PBS containing 50% glycerol 应用 应用 This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Not recommended for IP. 生物信息 免疫原品种 Recombinant GRB2/Ash 克隆 clone 3F2 宿主 Mouse 特异性 N-terminal SH3 region of GRB2 同种型 IgG1κ 品种反应性 RatBovineHumanHamsterMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002086
Entrez基因汇总 The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. 基因符号 - Grb3-3
- GRB2
- EGFRBP-GRB2
- HT027
- ASH
- MST084
- MSTP084
纯化方法 Protein A chromatography UniProt编号 - P62993
UniProt汇总 FUNCTION: SwissProt: P62993 # Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
SIZE: 217 amino acids; 25206 Da
SUBUNIT: Associates with activated Tyr-phosphorylated EGF receptors and PDGF receptors via its SH2 domain. Also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, SHC and LNK; probably via the concerted action of both its SH2 and SH3 domains. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Interacts with phosphorylated TOM1L1 and MET. Interacts with the phosphorylated C-terminus of SH2B2. Interacts with phosphorylated SIT1, LAX1, LAT, LAT2 and LIME1 upon TCR and/or BCR activation. Interacts with PTPNS1, REPS2 and the syntrophin SNTA1. Interacts with REPS1 and PIK3C2B via its SH3 domains (By similarity). Interacts with HCV NS5A via its SH3 domains. Interacts with CBL and CBLB. Interacts with SHB, INPP5D/SHIP1, SKAP1 and SKAP2.
DOMAIN: SwissProt: P62993 The SH3 domains mediate interaction with SHB.
SIMILARITY: Belongs to the GRB2/sem-5/DRK family. & Contains 1 SH2 domain. & Contains 2 SH3 domains.产品使用声明 质量保证 routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract 销售限制 This product is not available for sale in Japan. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 100 µg
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