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品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1482 描述 Anti-GβL/mLST8 Antibody, clone 3E1.2 Alternate Names - Target of rapamycin complex subunit LST8
- G protein beta subunit-like
- Protein GbetaL
- TORC subunit LST8
背景信息 GβL (G protein beta protein subunit-like), also known as mLST8, is a member of the mammalian Target of Rapamycin (mTOR) complex. The complex composed of mTOR, regulatory associated protein of mTOR (Raptor) and mammalian LST8/G-protein β-subunit like protein (mLST8/GβL) is responsible for regulating cell growth and nutrient and growth factor-mediated mTOR signaling. 产品信息 格式 Purified 控制 - HEK293 cell lysate
演示 Purified mouse ascites in buffer containing
0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
应用 应用 This Anti-GβL/mLST8 Antibody, clone 3E1.2 is validated for use in WB, IC for the detection of GβL/mLST8. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 生物信息 免疫原品种 GST-tagged recombinant human GβL/mLST8. 克隆 3E1.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes GβL/mLST8. 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Proven to react with human and mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_071767.3
基因符号 - mLST8
- Gable
- LST8
- MGC111011
- OTTHUMP00000159046
- POP3
- WAT1
纯化方法 Protein G purfied UniProt编号 - Q9BVC4
UniProt汇总 FUNCTION: Unessential component of the TORC1 complex and essential component of the TORC2 complex. TORC1 controls cell growth in response to environmental signals, and is inactivated by nutrient limitation and environmental stress. Within TORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RAPTOR interaction and favors RAPTOR-mediated inhibition of FRAP1 activity. TORC2 controls polarity of the actin cytoskeleton via the RAC1 pathway. TORC2 mediates phosphorylation of Akt/PKB on 'Ser-473' and phosphorylation of PKCalpha on 'Ser-657'.
SUBUNIT STRUCTURE: Interacts with FRAP1, RAPTOR and RHEB. Part of the target of rapamycin complex 1 (TORC1) which contains LST8, FRAP1, RAPTOR and AKT1S1. TORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the target of rapamycin complex 2 (TORC2) which contains FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP12-rapamycin.
SUBCELLULAR LOCATION: Cytoplasm By similarity.
TISSUE SPECIFICITY: Broadly expressed, with highest levels in skeletal muscle, heart and kidney. Ref.6
SEQUENCE SIMILARITIES: Belongs to the WD repeat LST8 family.
Contains 7 WD repeats.
产品使用声明 质量保证 Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected GβL/mLST8 in HEK293 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C for 1 year from date of receipt. 包装信息 数量 100 µg
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