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merck millipore,默克密理博,05-1324,Anti-phospho-Histone H1 Antibody, clone 12D11
产品名称：Anti-phospho-Histone H1 Antibody, clone 12D11
产品型号：05-1324
Use Anti-phospho-Histone H1 Antibody, clone 12D11 (Mouse Monoclonal Antibody) validated in IHC, ICC, IP, WB to detect phospho-Histone H1 also known as Histone H1a, histone 1 H1b.
merck millipore,默克密理博,05-1324,Anti-phospho-Histone H1 Antibody, clone 12D11
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Vrt, H, B, M WB, IHC, ICC M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 05-1324 描述 Anti-phospho-Histone H1 Antibody, clone 12D11 Alternate Names - histone cluster 1, H1b
- H1 histone family, member 5
- Histone H1a
- histone 1, H1b
- histone 1
- H1b
背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures. 产品信息 格式 Culture Supernatant 控制 - HeLa cell lysate, treated with colcemid.
演示 Supplied as 200 μL of culture supernatant with 0.05% sodium azide. 应用 应用 Use Anti-phospho-Histone H1 Antibody, clone 12D11 (Mouse Monoclonal Antibody) validated in IHC, ICC, IP, WB to detect phospho-Histone H1 also known as Histone H1a, histone 1 H1b. 主要应用 - Western Blotting
- Immunohistochemistry
- Immunocytochemistry
应用说明 Immunohistochemistry: This antibody has been reported by an independent laboratory to detect CDK (cyclin dependent kinase) phosphorylated Histone H1 in benign tissue sections.1
Immunocytochemistry:
This antibody has been demonstrated by an outside laboratory to show positive immunostaining for phosphorylated histone H1 in HUSK (human fetal primary myoblasts) cells fixed with 3.7% paraformaldehyde followed by permeabilization with 0.2% Triton X-100.2
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to immunoprecipitate phosphorylated histone H1 using solid phase beads bound to anti-mouse IgM.
生物信息 免疫原品种 The immunogen was KLH-conjugated bovine nuclear extract. 克隆 12D11 宿主 Mouse 特异性 Recognizes phosphorylated Histone H1, Mr 32 kDa. 同种型 IgMκ 品种反应性 VertebratesHumanBovineMouse Species Reactivity Note Human, mouse, bovine. Broad species cross-reactivity is expected, based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005319
Entrez基因汇总 Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is
wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].
基因符号 - OTTHUMP00000017748
- H1
- H1.5
- H1F5
- MGC126630
- MGC126632
修改 - Phosphorylation
UniProt编号 - P16401
UniProt汇总 FUNCTION: Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: Belongs to the histone H1/H5 family.
产品使用声明 质量保证 Western Blot Analysis:
1:1000 dilution of this antibody detected Histone H1 in colcemid-treated HeLa lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 200 µL
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下一件merck millipore产品：merck millipore,默克密理博,AB4344,Anti-LYAR Antibody (Ly1 Antibody reactive clone)
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