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merck millipore,默克密理博,05-1232,Anti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2
产品名称：Anti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2
产品型号：05-1232
Anti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2 is a mouse monoclonal antibody for detection of acetyl Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16), HIST1H4 & has been valid More>>
merck millipore,默克密理博,05-1232,Anti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1232 商名 - Upstate
描述 Anti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2 Alternate Names - HIST1H4
- H4K16Ac
- Histone H4 (acetyl K16)
背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Global loss of acetylation at Lys16 is a common hallmark of human tumor cells, suggesting a role for acetylated Lys16 in preventing cell transformation. Moreover, Lys16 is the only histone mark that has been directly linked to chromatin refolding and maintainence of chromatin structure. The Sir3 protein helps form telomeric heterochromatin by interacting with hypoacetylated histone H4 Lys16 while the MYST-like acetyltransferase Sas2p is required for the acetylation H4-Lys16 in euchromatin. 产品信息 格式 Purified 控制 - Sodium butyrate-treated HeLa cell acid extracts.
演示 Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in PBS, 0.05% sodium azide. 应用 应用 Anti-acetyl Histone H4 (Lys16) Antibody, clone 4E10.2 is a mouse monoclonal antibody for detection of acetyl Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16), HIST1H4 & has been validated in WB, PIA. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic peptide containing the sequence (GKGGA[acetyl-K]RHRKC, corresponding to the sequence surrounding acetylated lysine 16 of human histone H4. 表位 Acetyl Lys16 克隆 4E10.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes histone H4 acetylated on Lys16, Mr 11 kDa. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Based on sequence homology, broad species cross-reactivity is expected. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_003529
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq].
修改 - Acetylation
纯化方法 Protein G purfied UniProt编号 - P62805
UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Subcellular location: Nucleus.
Post-translational modification: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.
产品使用声明 质量保证 Routinely evaluated by Western Blot
Western Blot Analysis:
A 0.1- 1 μg/mL dilution of a recent lot detected acetylated histone H4 (Lys16) in acid extracts of sodium butyrate-treated HeLa cells. The antibody did not detect unmodified recombinant Histone H3 (Catalog # 14-411)使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
包装信息 数量 100 µg
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