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品种反应性 主要应用 宿主 格式 抗体类型 R, M, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1070 描述 Anti-PRAS40 Antibody, clone 9D10.2 Alternate Names - proline-rich Akt substrate, 40 kDa
- 40 kDa proline-rich AKT substrate
- AKT1 substrate 1 (proline-rich)
背景信息 PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate, as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to, and regulate mTORC1 (mTOR, Raptor, mLST8) kinase activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it to bind Raptor of mTORC1 in the absence of insulin1. It is thought that PRAS40 inhibits the kinase activity of mTORC1, when bound to Raptor, by preventing binding to its substrates such as p70S6K and 4EBP1. 产品信息 格式 Purified 控制 - NIH/3T3 cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 This Anti-PRAS40 Antibody, clone 9D10.2 is validated for use in WB, IP, IC for the detection of PRAS40. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: 1 µg/mL from a previous lot detected PRAS40 on 10 µg of HeLa and PC12 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated PRAS40 from 100 µg of NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected PRAS40 in NIH/3T3 cells.生物信息 免疫原品种 Recombinant protein corresponding to human PRAS40. 表位 N/A 克隆 9D10.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes PRAS40. 同种型 IgG2bκ 品种反应性 RatMouseHuman Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001092102.1
Entrez基因汇总 AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]. 基因符号 - PRAS40
- AKT1S1
- Lobe
纯化方法 Protein G purfied UniProt编号 - Q96B36
UniProt汇总 FUNCTION: May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SIZE: 256 amino acids; 27383 Da
SUBUNIT: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity).
TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).产品使用声明 质量保证 Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected PRAS40 on 10 µg of NIH/3T3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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