merck millipore,默克密理博,04-953,Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

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merck millipore,默克密理博,04-953,Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
产品名称：Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
产品型号：04-953
Detect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.

merck millipore,默克密理博,04-953,Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-953,Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

Replacement Information
Replacement Information	Cat. # 05-953. 05-953 will only be availabe on a custom basis.

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Zebrafish, Ca, H, M	WB	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-953
Replaces	05-953
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
背景信息	SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

产品信息
格式	Culture Supernatant
控制	HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ
演示	Cultured supernantant in 0.05% sodium azide

应用
应用	Detect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2
克隆	A5S
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.
同种型	IgG
品种反应性	ZebrafishCanineHumanMouse
Species Reactivity Note	: Predicted to cross-react with X. laevis based on sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001003652.2 NM_005901.4
Entrez基因汇总	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
基因符号	JV18-1 SMAD2 hMAD-2 MGC22139 JV18 MADH2 MGC34440 hSMAD2 MADR2
修改	Phosphorylation
UniProt编号	Q15796
UniProt汇总	FUNCTION: SwissProt: Q15796 # Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma. SIZE: 467 amino acids; 52306 Da SUBUNIT: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2- alpha subunit, CREB-binding protein (CBP), p300, SKI, SNON and SMURF2. Interacts with AIP1 and HGS. Interacts with NEDD4L in response to TGF-beta (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. TISSUE SPECIFICITY: Expressed at high levels in skeletal muscle, heart and placenta. PTM: Phosphorylated on one or several of Thr-220, Ser-245, Ser- 250, and Ser-255. In response to TGF-beta, phosphorylated on Ser- 465/467 by TGF-beta and activin type 1 receptor kinases. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. & In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation (By similarity).DISEASE:SwissProt: Q15796 # Defects in SMAD2 are found in sporadic cases of colorectal carcinoma. SIMILARITY: SwissProt: Q15796 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain.

产品使用声明
质量保证	Routinely evaluated by immunoblot analysis.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.