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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-900 Replaces 05-900 描述 Anti-phospho-Met (Tyr1234/1235) Antibody, clone NL8 | 04-900 Alternate Names - oncogene MET
- HGF receptor
- HGF/SF receptor
- Met proto-oncogene tyrosine kinase
- SF receptor
- Scatter factor receptor
- met proto-oncogene
- met proto-oncogene (hepatocyte growth factor receptor)
背景信息 The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target. 产品信息 格式 Purified 控制 - RIPA lysates from A431 cells co-expressing Met/HGFR and HGF.
演示 Cultured supernantant containing 0.05% sodium azide. 应用 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic peptide (…KE[pY][pY}SV…) in which pYpY corresponds to phosphorylated amino acids 1234 and 1235 of human Met/HGFR. 表位 pTyr1234/1235 克隆 NL8 浓缩 Cultured supernantant containing 0.05% sodium azide. 宿主 Rabbit 特异性 Recognizes phosphorylated Met, Mr 140 kDa. Cross-reacts with phosphorylated EGFR. 同种型 IgG 品种反应性 Human Species Reactivity Note Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000236.2
Entrez基因汇总 The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various
mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants
encoding different isoforms have been found for this gene.基因符号 - c-Met
- AUTS9
- EC 2.7.10.1
- HGFR
- OTTHUMP00000069168
- RCCP2
修改 - Phosphorylation
UniProt编号 - P08581
UniProt汇总 FUNCTION: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 By similarity. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN:The kinase domain is involved in SPSB1 binding.
INVOLVEMENT IN DISEASE: Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC)
Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance.
Genetic variations in MET may be associated with susceptibility to autism type 9 (AUTS9) [MIM:611015]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain.
产品使用声明 质量保证 Western Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-Met in RIPA lysates from NIH-3T3 cells co-expressing Met/HGFR and HGF. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance包装信息 数量 100 µl
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