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merck millipore,默克密理博,04-886,Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal
产品名称：Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal
产品型号：04-886
Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB.
merck millipore,默克密理博,04-886,Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal
- 产品介绍
- merck millipore,默克密理博,04-886,Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Chp, R, H, M, B WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-886 Replaces 05-886 描述 Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal Alternate Names - transcription factor STAT5B
- signal transducer and activator of transcription 5A
- signal transducer and activator of transcription 5B
背景信息 The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation. 产品信息 格式 Culture Supernatant 控制 - PDGF treated 3T3/NIH cells
演示 Cultured supernatant containing 0.05% sodium azide. 应用 应用 Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB. 主要应用 - Western Blotting
不建议的应用 - Immunoprecipitation
生物信息 免疫原品种 KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B. 表位 pTyr694/699 克隆 A11W 宿主 Rabbit 特异性 Phospho-STAT5A/B (Tyr694/699). 同种型 IgG 品种反应性 ChimpanzeeRatHumanMouseBovine 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003152 (STAT5A), NM_012448 (STAT5B)
Entrez基因汇总 The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq]
基因符号 - STAT5
- MGF
修改 - Phosphorylation
UniProt编号 - P42229
UniProt汇总 FUNCTION: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1 and SOCS7.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note= Translocated into the nucleus in response to phosphorylation.
PTM: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity.
SIMILARITY: Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
产品使用声明 质量保证 Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µL
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