merck millipore,默克密理博,04-817,Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

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merck millipore,默克密理博,04-817,Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal
产品名称：Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal
产品型号：04-817
Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified u More>>

merck millipore,默克密理博,04-817,Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-817,Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ChIP, WB, ICC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-817
Replaces	05-817
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal
Alternate Names	H3 histone, family 3B (H3.3B) H3 histone, family 3A, H3S10P Histone H3 (phospho S10) H3 histone, family 3A H3 histone, family 3B family 3B
背景信息	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine

产品信息
格式	Culture Supernatant
控制	UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts
演示	Cultured supernantant in 0.05% sodium azide.

应用
应用	Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified using DB & Mplex, published in peer reviewed journals & validated in ChIP, IF, WB, ICC, ChIP-seq.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Western Blot Analysis: A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A). Beadlyte® Histone-Peptide Specificity Assay: A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected. Immunofluorescence: Representative image from a previous lot. Positive chromosome immunostaining for mitotic HeLa cells and A431 cells. Chromatin Immunoprecipitation: A previous lot was shown to be suitable for ChIP by an independent laboratory.

生物信息
免疫原品种	Peptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3
克隆	MC463
宿主	Rabbit
特异性	Histone H3 phosphorylated at Serine 10
同种型	IgG
品种反应性	Human
Species Reactivity Note	Broad species cross-reactivity is expected
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003493
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
基因符号	MGC126886 HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ HIST3H3 H3FT H3T
修改	Phosphorylation
UniProt编号	Q16695
UniProt汇总	FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells. DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity). SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.

产品使用声明
质量保证	routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.