- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
- merck millipore,默克密理博,04-797,Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-797 Replaces 05-797 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39 Alternate Names - protein tyrosine kinase ERK2
- Mitogen-activated protein kinase
- Extracellular signal-regulated kinase
背景信息 Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively). 产品信息 格式 Purified 控制 - RIPA cell lysate from NGF stimulated PC-12 cells
演示 Cultured supernantant in 0.05% sodium azide 应用 主要应用 - Western Blotting
应用说明 Western Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte® Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex® microspheres. (Figure B).
生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species. 克隆 Aw39 浓缩 Variable 宿主 Rabbit 特异性 Recognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2). 同种型 IgG 品种反应性 RatHuman Species Reactivity Note Human and rat. Predicted to cross-react with many species based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002745, NM_002746
Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. 基因符号 - ERT1
- MAPK1
- p38
- ERK
- P42MAPK
- ERK2
- p40
- PRKM1
- p41mapk
- PRKM2
- MAPK2
- p42-MAPK
- p41
- ERK-2
修改 - Phosphorylation
UniProt编号 - P28482
UniProt汇总 FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,197332,Bcl-2 Inhibitor III, EM20-25 - CAS 141266-44-6 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,557502,Ro-20-1724 - CAS 29925-17-5 - Calbiochem
相关产品
merck millipore,默克密理博,234140,Collagenase Inhibitor I - Calbiochem
merck millipore,默克密理博,6500-OP,OmniPur PBS Tablets - Calbiochem
merck millipore,默克密理博,1125-OP,OmniPur Acridine Orange - CAS 10127-02-3 - Calbiochem
merck millipore,默克密理博,MAB8002,Anti-Hepatitis E Virus Antibody, aa 434-457, clone 1E6
merck millipore,默克密理博,197332,Bcl-2 Inhibitor III, EM20-25 - CAS 141266-44-6 - Calbiochem
merck millipore,默克密理博,557502,Ro-20-1724 - CAS 29925-17-5 - Calbiochem
merck millipore,默克密理博,128136,7α, 25-dihydroxycholesterol - Calbiochem
merck millipore,默克密理博,0500-3115,Muse Cell Analyzer
merck millipore,默克密理博,AB1961,Anti-Dynein Antibody, Left
merck millipore,默克密理博,GR11L,Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)