merck millipore,默克密理博,04-436,Anti-Bcl-2 Antibody, rabbit monoclonal

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merck millipore,默克密理博,04-436,Anti-Bcl-2 Antibody, rabbit monoclonal
产品名称：Anti-Bcl-2 Antibody, rabbit monoclonal
产品型号：04-436
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Bcl-2 using this Anti-Bcl-2 Antibody, Rabbit More>>

merck millipore,默克密理博,04-436,Anti-Bcl-2 Antibody, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-436,Anti-Bcl-2 Antibody, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB, FC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-436
品牌系列	Upstate
商名	Upstate
描述	Anti-Bcl-2 Antibody, rabbit monoclonal

产品信息
格式	Purified
演示	In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Bcl-2 using this Anti-Bcl-2 Antibody, Rabbit validated for use in FC, IF, IP, WB, IH(P).
主要应用	Western Blotting Flow Cytometry

生物信息
免疫原品种	KLH-conjugated synthetic peptide between residues BH3 and BH4 of human Bcl-2.
宿主	Rabbit
特异性	Recognizes a region between residues BH3 and BH4 of human Bcl-2.
同种型	IgG
品种反应性	MouseHuman
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_000657.2 NM_000633.2
Entrez基因汇总	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
基因符号	Bcl-2 BCL2
UniProt编号	P10415
UniProt汇总	FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). SIZE: 239 amino acids; 26266 Da SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. TISSUE SPECIFICITY: Expressed in a variety of tissues. DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1. PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. SIMILARITY: Belongs to the Bcl-2 family.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

包装信息
数量	100 µL

merck millipore,默克密理博,04-436,Anti-Bcl-2 Antibody, rabbit monoclonal

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