merck millipore,默克密理愽
  • merck millipore,默克密理博,04-1522,Anti-CAF1 p150 Antibody, clone SS1, 1-3

  • merck millipore,默克密理博,04-1522,Anti-CAF1 p150 Antibody, clone SS1, 1-3

    产品名称：Anti-CAF1 p150 Antibody, clone SS1, 1-3
    产品型号：04-1522
    Anti-CAF1 p150 Antibody, clone SS1, 1-3 is a Mouse Monoclonal Antibody for detection of CAF1 p150 also known as Chromatin assembly factor I p150 subunit & has been validated in WB, ICC & IP.

    merck millipore,默克密理博,04-1522,Anti-CAF1 p150 Antibody, clone SS1, 1-3

  • 产品介绍
  • merck millipore,默克密理博,04-1522,Anti-CAF1 p150 Antibody, clone SS1, 1-3

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HICC, WBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 04-1522
    描述 Anti-CAF1 p150 Antibody, clone SS1, 1-3
    Alternate Names
    • chromatin assembly factor I (150 kDa)
    • chromatin assembly factor 1, subunit A (p150)
    • Chromatin assembly factor I p150 subunit
    • CAF-I 150 kDa subunit
    • CAF-1 subunit A
    背景信息 Chromatin assembly factor (CAF), is a heterotrimeric complex that belongs to the histone chaperone family. It is found in yeast, animals and plants, and facilitates the assembly of chromatin during DNA replication and repair. There are three CAF subunits: p48, p60, and p150. The p150 subunit interacts with heterochromatin protein 1, methyl-binding protein
    1 and the Bloom’s syndrome DNA-helicase BLM. Mutations in CAF p150 have resulted in defects in embryo development.
    产品信息
    格式 Purified
    控制
    • HeLa cell lysate
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 Anti-CAF1 p150 Antibody, clone SS1, 1-3 is a Mouse Monoclonal Antibody for detection of CAF1 p150 also known as Chromatin assembly factor I p150 subunit & has been validated in WB, ICC & IP.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Western Blot Analysis: 1 µg/ml from a representative lot detected CAF p150 on 10 µg of HeLa cell lysate.
    生物信息
    免疫原品种 His-tagged recombinant human protein corresponding to CAF-1 p150.
    表位 Unknown
    克隆 clone SS1, 1-3
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes CAF-1 p150.
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_005483
    Entrez基因汇总 Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578])
    基因符号
    • hp150
    • CHAF1A
    • CAF
    • CAF-1
    • CAF-Ip150
    • CAF1
    • CAF1B
    • CAF1P150
    • P150
    纯化方法 Protein G purfied
    UniProt编号
    • Q13111
    UniProt汇总 FUNCTION: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci By similarity. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

    SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.

    SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.

    DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.

    DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

    PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

    SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
    产品使用声明
    质量保证 Evaluated by Western Blot in HeLa cell lysate.

    Western Blot Analysis: 0.5 µg/ml of this antibody detected CAF on 10 µg of HeLa cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,04-1522,Anti-CAF1 p150 Antibody, clone SS1, 1-3

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