merck millipore,默克密理博,04-1140,Anti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1140,Anti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal
产品名称：Anti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal
产品型号：04-1140
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PTP1B Antibody, clone EP1841Y, Rabbit is an ant More>>

merck millipore,默克密理博,04-1140,Anti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1140,Anti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB, FC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1140
描述	Anti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal
Alternate Names	protein tyrosine phosphatase, placental Protein-tyrosine phosphatase 1B protein tyrosine phosphatase 1B protein tyrosine phosphatase, non-receptor type 1
背景信息	PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and is a drug discovery target in the fields of diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.

产品信息
格式	Unpurified
控制	HeLa, HepG2, and Jurkat cell lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PTP1B Antibody, clone EP1841Y, Rabbit is an antibody against PTP1B for use in WB, FC, IH(P) & IC.
主要应用	Immunocytochemistry Western Blotting Flow Cytometry
应用说明	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected PTP1B in human tonsil tissue. Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC. Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.

生物信息
免疫原品种	Synthetic peptide corresponding to residues surrounding Ser386 of human PTP1B.
表位	Ser386
克隆	EP1841Y
宿主	Rabbit
特异性	This antibody detects both phosphorylated and nonphosphorylated PTP1B.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002818
Entrez基因汇总	The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
基因符号	PTP1B EC 3.1.3.48 OTTHUMP00000031266 PTP-1B
纯化方法	Unpurified
UniProt编号	P18031
UniProt汇总	FUNCTION: May play an important role in CKII- and p60c-src-induced signal transduction cascades By similarity. CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa, HepG2, and Jurkat cell lysates. Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect PTP1B in HeLa, HepG2, and Jurkat cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.