merck millipore,默克密理博,04-1130,Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal

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merck millipore,默克密理博,04-1130,Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal
产品名称：Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal
产品型号：04-1130
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1130,Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1130,Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	FC, WB, ICC, IHC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1130
描述	Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal
Alternate Names	T cell-specific transcription factor 1-alpha lymphoid enhancer-binding factor 1
背景信息	Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate, and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.

产品信息
格式	Unpurified
控制	Jurkat cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LEF-1/TCF-1 α in human colon adenocarcinoma tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in IC. Flow Cytometry: A 1:20 dilution of a representative lot was used in flow cytometry.

生物信息
免疫原品种	Synthetic peptide corresponding to human LEF-1/TCF-1 α.
克隆	EP2030Y
宿主	Rabbit
特异性	This antibody recognizes LEF-1/TCF-1 α. Recognition mpas to an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_016269.2
基因符号	TCF1-alpha LEF-1 TCF1ALPHA
纯化方法	Unpurified
UniProt编号	Q9UJU2

产品使用声明
质量保证	Evaluated by Western Blot on Jurkat cell lysates. Western Blot Analysis: A 1:25,000-50,000 dilution of this antibody was used to detect LEF-1/TCF-1 α in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.