merck millipore,默克密理博,04-1021,Anti-ATF-2 (CT) Antibody, clone E243, rabbit monoclonal

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merck millipore,默克密理博,04-1021,Anti-ATF-2 (CT) Antibody, clone E243, rabbit monoclonal
产品名称：Anti-ATF-2 (CT) Antibody, clone E243, rabbit monoclonal
产品型号：04-1021
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-ATF-2 (C-term) Antibody, clone E243 is a Rabbit More>>

merck millipore,默克密理博,04-1021,Anti-ATF-2 (CT) Antibody, clone E243, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1021,Anti-ATF-2 (CT) Antibody, clone E243, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1021
描述	Anti-ATF-2 (CT) Antibody, clone E243, rabbit monoclonal
Alternate Names	cAMP-responsive element-binding protein 2 Cyclic AMP-responsive element-binding protein 2 activating transcription factor 2 activating transcription factor 2 splice variant ATF2-var2 cAMP response element-binding protein CRE-BP1 cAMP responsive element binding protein 2 cAMP responsive element binding protein 2, formerly cAMP-dependent transcription factor ATF-2
背景信息	The ATF/CREB family of DNA binding proteins consists of transcription factors whose activity is regulated by specific signaling pathways, including the stress/cytokine/radiation inducible pathways that function through SAPK1/JNK activation. Two threonine residues at positions 69 and 71 in the N-terminal region of ATF-2 are essential in mediating adenovirus E1A-inducible transcriptional activation. These residues are efficiently phosphorylated in vivo and are excellent substrates for SAPK1/JNK in vitro.

产品信息
格式	Unpurified
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-ATF-2 (C-term) Antibody, clone E243 is a Rabbit Monoclonal Antibody for detection of ATF-2 has been validated in WB, ICC, IHC(P) & IP.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected ATF-2 in human breast carcinoma tissue. Immunocytochemistry Analysis: A 1:250-500 dilution from a previous lot was used in IC. Immunoprecipitation Analysis: A 1:60 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in C-terminus of human ATF-2.
表位	C-terminus
克隆	E243
宿主	Rabbit
特异性	The antibody does not cross-react with other ATF family members.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001880
基因符号	CRE-BP1 CREB-2 CREB2 CREBP1 HB16 MGC111558 OTTHUMP00000163262 TREB7
纯化方法	Unpurified
UniProt编号	P15336
UniProt汇总	FUNCTION: Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex. SUBUNIT STRUCTURE: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Abundant expression seen in the brain. PTM: Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. SEQUENCE SIMILARITIES: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger. CAUTION: It is uncertain whether Met-1 or Met-19 is the initiator.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa cell lysates. Western Blot Analysis: A 1:10,000 dilution of this antibody was used to detect ATF-2 in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.