pall,颇尔,PRC 层析预装柱,PRC05X050QHCEL01, PRC05X050SHCEL01, PRC05X050QCHDF01, PRC05X050CMCHDF01, PRC08X100QHCEL01, PRC08X100SHCEL01, PRCSTARAX1ML, PRCSTARAX5ML, PRC05X050MEPHCEL01, PRC05X050HEAHCEL01, PRC05

pall,颇尔,PRC 层析预装柱

For Ion Exchange and Mixed-Mode Chromatography Laboratory Applications

• Convenient – Ready-to-use 1 and 5 mL pre-packed columns.
• Easy to use – Direct connection to commonly-used laboratory chromatography systems, such as ÄKTA^♦ systems.
• Consistent – Screen 颇尔 ion exchange and mixed-mode chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.
• Versatile – Two or more columns can be connected in series to increase the column height and more closely model real pilot scale conditions or scale-down applications.

离子交换层析

Q and S HyperCel™ sorbents are composed of a rigid cellulose matrix that has excellent flow properties and generates low back pressure.These sorbents provide:

• High dynamic binding capacity at short residence time.
• Either capture or intermediate step use.
• Fast re-equilibration allowing buffer and time savings.
• Direct scale-up to pilot or production scale columns.

HyperCel STAR AX sorbent is an industry-scalable anion exchange chromatography sorbent designed for high productivity protein capture at moderate to physiological or higher salt conductivity (2 to 15 mS/cm), typical of undiluted biological feedstocks (i.e., mammalian cell culture supernatants, E. coli feedstock, plasma, others…).

• High dynamic binding capacity (DBC) at short residence time (2 minutes or lower)
• Direct capture of protein from undiluted feedstock at moderate or high conductivity
• Excellent flow rate properties for fast feedstock processing
• Distinctive selectivity, consistent in a broad conductivity range (2-15 mS/cm)
• Enhanced process economics

Q and CM Ceramic HyperD® sorbents have a high capacity hydrogel polymerized within the gigapores of a rigid ceramic bead.Typical features include:

• Good dynamic binding capacity independent of flow rate.
• Direct capture with high binding capacity for IgG at conductivities of 10-15 mS/cm on CM Ceramic HyperD sorbent.

Ceramic HyperD and HyperCel sorbents have different selectivities.The PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.

应用

• Native and recombinant proteins
• Plasmids
• 疫苗
• Monoclonal and polyclonal antibodies
• 血液制品
• 生物制药

混合式层析

MEP, HEA, and PPA HyperCel sorbents exploit unique selectivities of robust synthetic ligands to capture proteins or separate them from contaminants.They provide a unique separation mechanism different from conventional methods.All ligands operate predominantly by hydrophobic interaction.This "HIC-like" interaction typically takes place without the addition of lyotropic salt.

应用

• MEP HyperCel 填料
  • Antibody capture
  • Alternative to conventional hydrophobic interaction
• MEP, HEA, and PPA HyperCel sorbents
  • No salt/low salt alternative to hydrophobic interaction

材料成分

Body and End Caps:Molded polypropylene
17 μm Frit:PP/PE (polypropylene/polyethylene)

Outer Dimensions

1 mL:10 x 100 mm
5 mL:11.5 x 140 mm

Connections

Built-in 10-32 fittings

For HPLC/MPLC/ÄKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings.For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.

最大操作压力

1 mL:20 barg (290 psig)
5 mL:30 barg (435 psig)

Volume

1 mL:5 mm ID x 50 mm
5 mL:8 mm ID x 100 mm

Storage Solution

HyperCel STAR AX and Q/S HyperCel Sorbents:30% isopropanol/100 mM sodium phosphate, pH 4.3
Ceramic HyperD F Sorbent:20% ethanol/150 mM NaCl

Working Pressure

HyperCel STAR AX and Q/S HyperCel Sorbents:< 0.5 barg (7 psig)
Ceramic HyperD F Sorbent:< 1.5 barg (22 psig)
Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.

离子交换	Chemistry	平均颗粒尺寸（微米）	Ionizable Groups (μEq/mL)	Dynamic Capacity (mg/mL)1
Q HyperCel	Quaternary amine	75	99-138	≥ 1602
S HyperCel	磺酸	75	59-84	≥ 1353
Q 陶瓷 HyperD F	Quaternary amine	50	≥ 250	≥ 854
CM Ceramic HyperD F	羧	50	≥ 250	≥ 605
HyperCel STAR AX	–	80 微米	–	>100 mg8 8 5 mg/mL BSA in 25 mM Tris-HCl, 0.14 M NaCl at 2 min residence time.

混合模式	配基	平均颗粒尺寸（微米）	Ligand Density (μmole/mL)	Dynamic Capacity (mg/mL)1
MEP HyperCel	4-mercaptoethyl- pyridine (pKa = 4.8)	90	80-125	≥ 206
HEA HyperCel	Hexylamine (aliphatic) (pKa = 8.0)	90	58-84	≥ 407
PPA HyperCel	Phenylpropyla mine (aromatic) (pKa = 8.0)	90	58-80	≥ 407

¹ Determined at 10% breakthrough using:
² 5 mg/mL BSA in 50 mM Tris-HCl, pH 8.5 at 2 minute residence time.
³ 5 mg/mL human IgG in 50 mM sodium acetate, pH 4.5 at 2 minute residence time.
⁴ 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h.
⁵ 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h.
⁶ 5 mg/mL human IgG in PBS, flow rate 60 cm/h.
⁷ 5 mg/mL BSA in PBS, flow rate 100 cm/h.

图 1

Separation of Cytochrome C, BSA, and Human Transferrin on a PRC Q Ceramic HyperD F Pre-Packed Column

Column:Q Ceramic HyperD F PRC pre-packed column (5 mm ID x 50 mm).体积：1 mL linear flow rate:150 cm/h.Equilibration and wash:50 mM Tris-HCl, pH 8.6. Load:100 μL (5 mg/mL cytochrome C, 20 mg/mL BSA, and 20 mg/mL human transferrin in equilibration buffer).Elution:50 mM

图 2

Separation of Model Proteins (Ovalbumin, ß-lactoglobulin, Cytochrome C, and Lysozyme) on 1 mL PRC S HyperCel Pre- Packed Columns and Scale Up on 5 mL Column, Constant Residence Time of 2 Minutes

Columns:S HyperCel PRC pre-packed columns of 1 mL (5 mm ID x 50 mm) and 5 mL (8 mm ID x 100 mm).Load:for 1 mL column – 100 μL ovalbumin (10 mg/mL), ß-lactoglobulin (10 mg/mL), cytochrome C (2.5 mg/mL), and lysozyme (5 mg/mL).For 5 mL column – 500 μL of same proteins.Equilibration:50 mM Na acetate, pH 4.5. Elution:50 mM Na acetate, pH 4.5 + 0.5 M NaCl by linear gradient from 0 to 100%.

图 3

Separation of Lysozyme, α-Chymotrypsinogen, Ovalbumin, and BSA on a PRC PPA HyperCel Pre-Packed Column

Column:PPA HyperCel PRC pre-packed column (5 mm ID x 50 mm).体积：1 mL linear flow rate:75 cm/h.Equilibration and wash:PBS, pH 7.4. Load:500 μL (lysozyme, α-chymotrypsinogen, ovalbumin, and BSA, all at 2 mg/mL in equilibration buffer).Elution:by pH step gradient in phosphate/citrate buffer, pH 7.0, 5.4, and 2.6.

• AcroSep™ Chromatography Columns – Pre-packed 1 mL chromatography columns for accelerated protein purification and analysis.
• LRC Chromatography Columns – Empty glass columns for preparative and process optimization applications.
• Acrodisc® Units with Mustang® Membrane – Disposable chromatography units with high binding capacities and fast flow rates.

Part Number	描述	Pkg	Price	Qty
PRC Pre-Packed Chromatography Columns with Ion Exchange Sorbents
PRC05X050QHCEL01	Q HyperCel sorbent, 5 x 50	1 毫升	Call
PRC05X050SHCEL01	S HyperCel sorbent, 5 x 50	1 毫升	Call
PRC05X050QCHDF01	Q Ceramic HyperD F sorbent, 5 x 50	1 毫升	Call
PRC05X050CMCHDF01	CM Ceramic HyperD F sorbent, 5 x 50	1 毫升	Call
PRC08X100QHCEL01	Q HyperCel sorbent, 8 x 100	5 毫升	Call
PRC08X100SHCEL01	S HyperCel sorbent, 8 x 100	5 毫升	Call
PRCSTARAX1ML	HyperCel STAR AX sorbent 5 x 50	1 毫升	Call
PRCSTARAX5ML	HyperCel STAR AX sorbent 8 x 100	5 毫升	Call
PRC Pre-Packed Chromatography Columns with Mixed-Mode Sorbents
PRC05X050MEPHCEL01	MEP HyperCel sorbent, 5 x 50	1 毫升	Call
PRC05X050HEAHCEL01	HEA HyperCel sorbent, 5 x 50	1 毫升	Call
PRC05X050PPAHCEL01	PPA HyperCel sorbent, 5 x 50	1 毫升	Call
PRC08X100MEPHCEL01	MEP HyperCel sorbent, 8 x 100	5 毫升	Call
PRC08X100HEAHCEL01	HEA HyperCel sorbent, 8 x 100	5 毫升	Call
PRC08X100PPAHCEL01	PPA HyperCel sorbent, 8 x 100	5 毫升	Call

pall,颇尔,PRC 层析预装柱