pall,颇尔,Nanosep 和 Nanosep MF 浓缩离心管,OD003C33, OD003C34, OD003C35, OD010C33, OD010C34, OD010C35, OD030C33, OD030C34, OD030C35, OD100C33, OD100C34, OD100C35, OD300C33, OD300C34, OD300C35, ODM02C33, ODM02

Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples

• Ensures rapid processing of samples 
• Typical recoveries are > 90%
• Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
• A wide range of MWCOs, color-coded for easy identification
• Constructed of low-binding polypropylene 
• Ultrasonically welded seals prevent bypass or seal failure
• Fits standard centrifuge rotors that accept 1.5 mL tubes

• Concentrate, purify, and desalt oligonucleotides, DNA, RNA, and proteins
• Clean up labeling and PCR reactions
• Isolate DNA from agarose gel slices
• Separate oligonucleotides and RNA from acrylamide gels
• Concentrate PCR products regardless of size with 30K device if primer removal is required

材料成分

• Nanosep 装置
    Filter Media：Omega (modified polyethersulfone) ultrafiltration membrane
    Sample Reservoir, Membrane Support Base, and Filtrate Receiver:聚丙烯
• Nanosep MF Devices
    Filter Media：Bio-Inert (modified nylon) and GHP (hydrophilic polypropylene) membranes
    Sample Reservoir, Membrane Support Base, and Filtrate Receiver:聚丙烯

有效过滤面积

• 0.3 cm²

尺寸

• Overall Length (Fully Assembled with Cap):4.5 cm (1.8 in.)

Capacities

• Maximum Sample Volume:500 µL
• Final Concentrate Volume:15 µL
• Filtrate Receiver Volume:500 µL
• Hold-up Volume (Membrane/Support):< 5 微升

操作温度范围

• 0 - 40 °C (32 - 104 °F)

pH 范围

• Nanosep Devices: 1 - 14
• Nanosep MF Devices: 3 - 14

Maximum Centrifugal Force

• 14,000 x g

Centrifuge

• Fits rotors that accept 1.5 mL tubes

Sanitization

• Provided non-sterile.May be sanitized by filtering 70% ethanol through the device prior to use.

DNA Recovery as a Function of Device MWCO

A 500 μL sample of a 100 μg/mL DNA fragment solution containing 50 and 500 bp double-stranded DNA fragments was centrifuged at 5,000 x g in Nanosep devices to a final volume of 50 μL.Recovered samples were quantitated using absorbance at 260 nm.The 100K device was able to differentiate between the sizes of the DNA fragments.

Omega™ Membrane Has the Lowest Non-Specific DNA Binding

A 500 μL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was centrifuged at 5,000 x g in a 100K Nanosep or competitive device.The retained DNA was recovered in 40 μL TE (10 mM Tris, 1 mM EDTA, pH 8).The remaining radioactivity in the device was counted in a scintillation counter.A value at 1000 CPM roughly corresponds to 1% of the total radioactive sample.Omega membrane has the lowest non-specific binding, resulting in highest DNA recoveries.In general, DNA molecular weights do not correspond well with the MWCO because DNA is a long linear molecule.Effective retention of DNA by an ultrafiltration membrane requires a reduction in g-force to 5,000 x g.Otherwise, DNA can be forced through many MWCO membranes regardless of size.

Centrifugal Device Spin Times

• 用于超滤的AcroPrep Advance 96 孔板
• AcroWell™ 96-well Membrane-bottom Plates with GHP Membrane
• AcroWell™ 96-well Membrane-bottom Plates with BioTrace™ PVDF and NT Membranes 
• Acrodisc® Syringe Filters with GHP Membrane & GH Polypro (GHP) Membrane Disc Filters
• Acrodisc® Syringe Filters with Nylon Membrane & Nylaflo™ Membrane Disc Filters
• Acrodisc® Unit with Mustang™ E Membrane
• Acrodisc® Unit with Mustang™ Q Membrane
• Acrodisc® Unit with Mustang™ S Membrane
• BioTrace NT 纯硝化纤维素转印膜
• BioTrace PVDF 转印膜
• Biodyne® Transfer Membranes
• DMSO-Safe Acrodisc® Sterile Syringe Filter
• Ion Chromatography (IC) Acrodisc® Syringe Filters & Supor® (PES) Membrane Disc Filters