pall,颇尔,HA Ultrogel 羟基磷灰石填料,24775-082, 24775-025, 24775-017, 24775-041

For the Separation of Proteins,
Peptides, and Nucleic Acids

• High binding capacity and high flow rates
• Structure provides excellent rigidity and stability to pH and ionic strength changes, as well as to high temperature
• Easy cleaning with sodium hydroxide
• Scalable from research and development to manufacturing

• Separation of basic proteins (Cytochrome C, Lysozyme, etc.)
• Separtaion of human serum proteins and plant proteins such as lectins, glycoproteins, transferases, kinases, and trehalases
• Separation of phosphate dependent proteins and exzymes as well as
  DNA-dependent enzymes
• Can be used to remove DNA and endotoxins

Average Particle Size

• 120 微米

Hydroxyapatite Content (Weight/Volume)

• 40%

Agarose (Weight/Volume)

• 4%

排阻限制

• > 5,000,000 Da

Capacity for Cytochrome C¹

• > 7 毫克/毫升

Capacity for BSA²

• < 7 毫克/毫升

Working pH

• 5 - 13 (Note:Do not use < pH 4.0.)

Storage Temperature

• 2 - 30 ºC (36 - 86 °F)
• 2 - 8 ºC (36 - 46 °F) after opening

¹Determined at 50% breakthrough using 5 mg/mL Cytochrome C diluted 50/50 in 10 mM PBS, pH 6.8
at 30 cm/h.
²Determined at 50% breakthrough using 1 mg/mL BSA diluted 50/50 in 10 mM PBS, pH 6.8 at 12.5 cm/h.

Separation of a Mixture of Ribonuclease and Phytohemagglutinins (PHA-EL)

Column:1.6 x 6.5 cm; Sample:1 mg of protein mixture composed of ribonuclease (MW 14,700) and PHA-ELs (Erythroagglutinating and lymphostimulating Phytohemagglutinin) (MW 128,000) from Phaseolus vulgaris, in 1 mL of 5 mM potassium phophate, pH 6.8; Elution gradient:5 mM to 500 mM potassium phosphate, pH 6.8; Flow rate:14.4 cm/h.

Separation of Trypsin and Chymotrypsin from a Porcine Pancreatic Enzyme Extract

Column:1.6 x 5 cm; Sample:30 mg protein in 1 mL of 5 mM phosphate buffer, pH 6.8; Gradient:5 to 200 mM sodium phosphate, ph 6.8; Flow rate:10cm/h; Temperature:10 °C; Histogram with broken line:trypsiin activity; Histogram with solid line:chymotrypsin activity.Spec.Ac.: specific activity in U/mg. tr:trypsin, ch:chymostrypsin.Trypsin activity was primarily found in the peak eluted by 50 mM phosphate where the chymotrypsin was eluted by 100 mM phosphate.The final yilad was approximately 50%.