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merck millipore,默克密理博,SF006,ESGRO Complete Accutase
产品名称：ESGRO Complete Accutase
产品型号：SF006
The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions More>>
merck millipore,默克密理博,SF006,ESGRO Complete Accutase
- 产品介绍
描述 产品目录编号 SF006 品牌系列 Chemicon®
商名 - Chemicon
描述 ESGRO Complete Accutase 概述 ESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products. 产品信息 演示 Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
1X Accutase™ enzymes in Dulbecco's PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.应用 应用 The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium. 主要应用 - Stem Cell Culture
应用说明 Cell Detachment:
1. Thaw Accutase™ to room temperature.
2. Wash plate, flask or beads with sterile PBS.
3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.
4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).
5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase™ may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE. 包装信息 数量 100 mL
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