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merck millipore,默克密理博,SF-164,HIF-1alpha; Human, Cyanine 3, RNA Detection Probe,SmartFlare
产品名称：HIF-1alpha; Human, Cyanine 3, RNA Detection Probe, SmartFlare
产品型号：SF-164
Use SmartFlare RNA Detection probes to measure gene expression in live cells. With an overnight incubation of SmartFlare HIF-1alpha, Human, Cyanine 3, you can detect HIF-1alpha RNA levels within your More>>
merck millipore,默克密理博,SF-164,HIF-1alpha; Human, Cyanine 3, RNA Detection Probe,SmartFlare
- 产品介绍
重要规格表
Entrez Gene Number Species Reactivity Species Reactivity Note NM_001243084.1 H NM_001530.3 描述 产品目录编号 SF-164 商名 - SmartFlare
描述 HIF-1alpha; Human, Cyanine 3, RNA Detection Probe | SmartFlare 背景信息 With SmartFlare™ RNA Detection probes, you can detect RNA in LIVE CELLS with ease. With just an overnight incubation of HIF-1alpha; Human, Cyanine 3, RNA Detection Probe you can detect the presence of HIF-1alpha within your cells.
HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis, the posttranslational modification by prolyl hydroxylation as a key regulatory event that targets HIF-alpha subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. The transcriptional complex is composed of an alpha-beta heterodimer; HIF-beta being a constitutive nuclear protein that dimerises with oxygen regulated HIF-alpha subunits. In normoxia, 4-hydroxylation of human HIF-alpha at Pro402 or Pro564 by a set of HIF prolyl hydroxylase isoenzymes (PHD 1-3) mediates HIF1-alpha recognition by von Hippel-Lindau ubiquitin ligase complex leading to its proteasomal destruction. In hypoxia (deprivation of oxygen), lack of hydroxylase activity enables HIF-alpha subunits to escape destruction and become transcriptionally active. Thus HIF hydroxylases provide a focus for understanding cellular responses to hypoxia and target for therapeutic manipulation. There are several HIF factors, which include HIF 1-alpha, HIF 1-beta, HIF 2-alpha. HIF 1-alpha is an 812 a.a. protein in rat and 836 a.a. long in mouse and human. A master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. It is ubiquitous in expression as cytoplasmic in normoxia, nuclear translocation in response to hypoxia.产品信息 格式 Cyanine 3 演示 Lyophilized 应用 应用 Use SmartFlare RNA Detection probes to measure gene expression in live cells. With an overnight incubation of SmartFlare HIF-1alpha, Human, Cyanine 3, you can detect HIF-1alpha RNA levels within your cells. 主要应用 - RNA Detection
生物信息 品种反应性 Human Species Reactivity Note NM_001530.3 Entrez基因编号 - NM_001243084.1
基因符号 - HIF-1alpha
产品使用声明 质量保证 SmartFlare Probes have been tested in a buffer system to detect the release of the fluorophore in the presence of a complimentary base pair sequence for each lot to confirm target specificity. SmartFlare Probes have also been tested in a cell model system and demonstrated increased fluorescence in cells expressing the target compared to a negative SmartFlare Scramble Control. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2–8°C in lyophilized format ONLY. Room temperature is required for reconstituted product. 包装信息 数量 250 reactions
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