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merck millipore,默克密理博,681505,Anti-CaV2.2 (851-867) Rabbit pAb
产品名称：Anti-CaV2.2 (851-867) Rabbit pAb
产品型号：681505
This Anti-CaV2.2 (851-867) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of CaV2.2 (851-867).
merck millipore,默克密理博,681505,Anti-CaV2.2 (851-867) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~210 kDa and the ~240 kDa forms of α1B-subunit of Cav2.2. Supplied with a control peptide. Note: The α-subunit is highly sensitive to proteases. Please refer to the product insert for proper sample preparation. 产品目录编号 681505 品牌系列 Calbiochem®
同义词 Anti-N-Type-Ca2+ Channel 应用数据 
Detection of rat CaV2.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:200). Detection: chemiluminescence.
Detection of mouse of CaV2.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV2.2 channel (red) appearing in Purkinje cells (arrows) and distributed diffusely in the molecular layer (Mol) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of CaV2.2 to cell bodies but not to dendrites in the molecular layer (Figure B). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:100). Detection: fluorescence.参考 参考 Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
Westenbroek, R.E., et al. 1992. Neuron 9, 1099.产品信息 形式 Lyophilized 配方 50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, pH. 7.4 and 40 µg control peptide lyophilized from PBS. 防腐剂 ≤0.1% sodium azide (antibody only) 应用 应用参考 Immunocytochemistry and Immunohistochemistry Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403. Immunoprecipitation Westenbroek, R.E., et al. 1992. Neuron 9, 1099. 主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
生物信息 免疫原 a synthetic peptide [(C)RHHRHRDRDKTSASTPA] corresponding to amino acids 851-867 of the α1B-subunit of rat brain voltage-gated calcium channels, conjugated to KLH 免疫原品种 Rat 宿主 Rabbit 同种型 IgG 品种 mouse, rat 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Dissolve the antibody in 50 µl dH₂O to make a 300 µg/ml stock solution, aliquot and freeze (-20°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Dissolve the peptide in 100 µl dH₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
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