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merck millipore,默克密理博,R001A,TaKaRa Taq™ Polymerase
产品名称：TaKaRa Taq™ Polymerase
产品型号：R001A
TaKaRa Taq is a recombinant, thermostable, 95 kDa DNA polymerase encoded by the DNA polymerase gene of the Thermus aquaticus YT-1 strain that has been cloned into Escherichia coli.
merck millipore,默克密理博,R001A,TaKaRa Taq™ Polymerase
- 产品介绍
描述 产品目录编号 R001A 品牌系列 Chemicon®
商名 - Chemicon
- Taq
描述 TaKaRa Taq™ Polymerase 概述 TaKaRa™ Taq is a recombinant, thermostable, 95 kDa DNA polymerase encoded by the DNA polymerase gene of the Thermus aquaticus YT-1 strain that has been cloned into Escherichia coli. It has been shown to have essentially the same characteristics as the native Taq DNA polymerase. The enzyme is supplied at a concentration of 5 units/μL with 10X PCR Reaction Buffer containing optimized MgCl2 and 2.5 mM dNTP Mix.
Unit definition: One unit is the amount of the enzyme that will incorporate 10 nmol of dNTP into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer.
Reaction mixture for unit definition:
25mM TAPs (pH9.3 at 25°C); 50mM KCl; 2mM MgCl2; 1mM 2-mercaptoethanol; 200μM each dATP, dGTP, dTTP; 100μM alpha P32-dCTP; 0.25mg/mL activated salmon sperm DNA产品信息 演示 Taq Storage Buffer:
20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1 mM Dtt, 0.5% Tween20, 0.5% Nonidet P-40, 50% Glycerol
Supplied 10X PCR Buffer:
1mL/vial
Component (10X): 100mM Tris-HCl (pH8.3), 500 mM KCl, 15mM MgCl2
Supplied dNTP Mixture:
800μL/vial
Concentration: 2.5mM of each dNTP, pH7-9, solved in water (sodium salts). Purity is > 98% for each dNTP.应用 应用 TaKaRa Taq is a recombinant, thermostable, 95 kDa DNA polymerase encoded by the DNA polymerase gene of the Thermus aquaticus YT-1 strain that has been cloned into Escherichia coli. 主要应用 - DNA Amplification
应用说明 DNA amplification by Polyermerase Chain Reaction (PCR)
DNA sequencing
PCR condition (an example)
When amplifying 1 kbp DNA fragment
30 cycles of : 94°C 30sec, 55°C 30sec, 72°C 1min. Note: Denaturation condition varies depending on a used thermal cycler and tube. It is recommednded for 10-30sec at 94°C , or 1-10sec at 98°C.
General reaction mixture for PCR (total 100μL)
TaKaRa™ (5units/μL) 0.5μL
10X PCR Buffer 10μL
dNTP Mixture (2.5mM each) 8μL
Template <1μg
Primer 1 20 - 100 pmol (final conc. 0.2 - 1.0 μM)
Primer 2 20 - 100 pmol (final conc. 0.2 - 1.0 μM)
Sterilized distilled water up to 100μL
Cool Start Method
Minimizes the amplification of non-specific bands in PCR and achieves more accurate amplification. This is a simpler method without the need for special enzymes nor additional reagents.
Protocol of Cool Start Method*:
1. Keep all reagents on ice until used.
2. Preaper the reaction mixture on ice. The adding order of reagents does not influence results. The result will also not be affected even when the mixture is left on ice 30 min. before thermal cycling.
3. Set thermal cycler ready to start with the designated program. No need to change your PCR conditions especially for Cool Start.
4. Set the tubes in the thermal cycle and start thermal cycling immediately.
* Japan patent 2576741 for Cool Start Method is ownded by Shimadzu Corporation.生物信息 浓缩 5 units/μL 纯度 Nicking activity, endonuclease and exonuclease activity were not detected after the incubation of 0.6μg of supercoiled pBR322 DNA, 0.6μg of lamda DNA or 0.6μg of lamda-HindIII digest with 10 units of this enzyme for 1 hour at 74°C. 来源 Escherichia coli carrying a plasmid that encodes the Thermus aquaticus DNA polymerase gene. 产品使用声明 质量保证 PCR test: Good performance of DNA amplification by PCR was confirmed by using lamda DNA as the template (amplified fragment: 8kbp). Good performance of DNA amplification of a single copy gene by PCR was also confirmed by using human placenta DNA as the template, and human p53-primers (amplified fragment: 2.9kbp). 销售限制 This product is only available for sale in the United States. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C. 包装信息 数量 250 units
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