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merck millipore,默克密理博,OP03L,Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
产品名称：Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
产品型号：OP03L
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).
merck millipore,默克密理博,OP03L,Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated p53. 产品目录编号 OP03L 品牌系列 Calbiochem®
参考 参考 El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Kastan, M.B., et al. 1992. Cell 71, 587.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
Harlow, E., et al. 1981. J. Virol. 39 861.产品信息 形式 Lyophilized 配方 Lyophilized from a volatile buffer, 100 µg BSA. 负控制 Normal skin tissue or SK-OV-3 cells 正控制 Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147) 防腐剂 None 应用 应用参考 Immunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579. 主要应用 Flow Cytometry
Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
生物信息 免疫原 partially purified mouse p53 免疫原品种 Mouse 表位 within amino acids 376-378 of human p53 克隆 PAb421 宿主 Mouse 同种型 IgG2a 品种 human, monkey, mouse, rabbit, rat 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 +2°C to +8°C
无需冷冻 No
特殊指南 Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.
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