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merck millipore,默克密理博,MABS76,Anti-PI3 kinase alpha Antibody, clone 2B12.1
产品名称：Anti-PI3 kinase alpha Antibody, clone 2B12.1
产品型号：MABS76
This Anti-PI3 kinase alpha Antibody, clone 2B12.1 is validated for use in WB for the detection of PI3 kinase alpha.
merck millipore,默克密理博,MABS76,Anti-PI3 kinase alpha Antibody, clone 2B12.1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M WB M Purified Monoclonal Antibody 描述 产品目录编号 MABS76 Replaces 04-403 描述 Anti-PI3 kinase alpha Antibody, clone 2B12.1 Alternate Names - PtdIns-3-kinase regulatory subunit p85-alpha
- Phosphatidylinositol 3-kinase regulatory subunit alpha
- PI3-kinase regulatory subunit alpha
- PI3K regulatory subunit alpha
- PtdIns-3-kinase regulatory subunit alpha
- Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
- PI3-kinase subunit p85-alpha
背景信息 Phosphoinositol-3-kinases (PI3 Kinase, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class I PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class I p110 catalytic domains. Class I(A) include α, β, γ and class I(B). They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3K generates phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus de-activating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI3 kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders. 产品信息 格式 Purified 控制 - NIH/3T3 cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-PI3 kinase alpha Antibody, clone 2B12.1 is validated for use in WB for the detection of PI3 kinase alpha. 主要应用 - Western Blotting
生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human PI3 kinase alpha. 克隆 2B12.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Mouse Species Reactivity Note Demonstrated to react with Mouse. Expected to react with Human based on immunogen design. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_852556
Entrez基因汇总 Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]. 基因符号 - GRB1
- PIK3R1
纯化方法 Protein G UniProt编号 - P27986
UniProt汇总 FUNCTION: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
SUBUNIT STRUCTURE: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C-terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1 and phosphorylated IRS4, as well as with NISCH and HCST. Interacts with FASLG.
TISSUE SPECIFICTY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
DOMAIN: The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ.
SEQUENCE SIMILARITIES: Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domains.产品使用声明 质量保证 Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PI3 kinase alpha on 10 µg of NIH/3T3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,407290,Anti-α-Interferon Mouse mAb (MMHA-2)
下一件merck millipore产品：merck millipore,默克密理博,662091,Ubiquitin Conjugating Enzyme 5a, GST-Fusion, Human, Recombinant, E. coli
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