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品种反应性 主要应用 宿主 格式 抗体类型 H, M ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABS17 描述 Anti-Ubch9 Antibody Alternate Names - Ubiquitin-protein ligase E3
- Ubiquitin-conjugating enzyme E2 E3
- UbcH9
- Ubiquitin carrier protein E3
- Ubiquitin-conjugating enzyme E2-23 kDa
背景信息 SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. UbcH9 is a SUMO conjugating enzyme that mediates the conjugation of SUMO-1 to a number of proteins including RanGAP1, IκBα and PML. It is known to interact directly with proteins that are modified by SUMOylation and may be important for substrate recognition. It appears that conjugation of SUMO-1 to IκBβ prevents subsequent ubiquitinylation and thus protects it from proteasomal degradation. Thus, while ubiquitinylation targets proteins for rapid degradation, SUMO-1 modification acts antagonistically to generate proteins resistant to degradation. 产品信息 格式 Purified 控制 - NIH/3T3 cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Ubch9 using this Anti-Ubch9 Antibody validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected Ubch9 in A431 and HeLa cells. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human Ubch9. 克隆 15F1.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG2bκ 品种反应性 HumanMouse Species Reactivity Note Demonstrated to react with Human and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_006348
Entrez基因汇总 The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. 基因符号 - UBCH9
- UBE2E3
- UBCE4
纯化方法 Protein G purified UniProt编号 - Q969T4
UniProt汇总 FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
SUBUNIT STRUCTURE: The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus. Interacts with NEDD4L.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner.
TISSUE SPECIFICITY: Belongs to the ubiquitin-conjugating enzyme family.产品使用声明 质量保证 Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Ubch9 in 10 µg of NIH/3T3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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