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描述 产品目录编号 08-145 品牌系列 Upstate
商名 - Upstate
描述 NEDD8 Protein 概述 NEDD8 protein is a recombinant protein produced in E. coli corresponding to the human sequence encompassing an N-terminal His6-tag within a leader sequence. NEDD8, ubiquitin-like molecule, shares ~60% identity with ubiquitin at the amino acid level that can be covalently conjugated (neddylation) to a limited number of cellular proteins in a fashion similar to that of ubiquitin. The C-terminus of pro-NEDD8 is efficiently processed to expose glycine76 in the mature form. This processing is required for conjugation to target proteins via a range of specific activating and conjugating enzymes. 产品信息 演示 100 µg of concentration 0.5 mg/mL in 20mM HEPES, pH8.0, 50mM NaCl, 1mM DTT, 10% glycerol. 生物信息 浓缩 0.5 mg/mL 纯度 ≥95% 品种 Human Entrez基因编号 - NM_006156.2
基因符号 - MGC104393
- NEDD8
- MGC125897
- Neddylin
- Nedd-8
- MGC125896
- FLJ43224
UniProt编号 - Q15843
UniProt汇总 FUNCTION: SwissProt: Q15843 # Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
SIZE: 81 amino acids; 9072 Da
SUBUNIT: Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.
SUBCELLULAR LOCATION: Nucleus. Note=Mainly nuclear.
TISSUE SPECIFICITY: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
PTM: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
SIMILARITY: SwissProt: Q15843 ## Belongs to the ubiquitin family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -80°C from date of shipment for up to 12 months. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80ºC. 包装信息 数量 100 µg
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