merck millipore,默克密理博,MABS160,Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, rabbit monoclonal

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merck millipore,默克密理博,MABS160,Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, rabbit monoclonal
产品名称：Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, rabbit monoclonal
产品型号：MABS160
This Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, Rabbit is validated for use in WB, IH, IP for the detection of phospho EZH2 (Thr487).

merck millipore,默克密理博,MABS160,Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABS160,Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	IHC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABS160
描述	Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, rabbit monoclonal
Alternate Names	Enhancer of zeste homolog 2 enhancer of zeste homolog 2 (Drosophila) Lysine N-methyltransferase 6 enhancer of zeste (Drosophila) homolog 2 enhancer of zeste 2 histone-lysine N-methyltransferase EZH2
背景信息	Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo (2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3).

产品信息
格式	Unpurified
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing 50% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	This Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, Rabbit is validated for use in WB, IH, IP for the detection of phospho EZH2 (Thr487).
主要应用	Immunohistochemistry Western Blotting
应用说明	Immunoprecipitation Analysis: A 1:10 dilution from a representative lot was used in IP. Immunohistochemistry Analysis: A 1:100-1:250 dilution from a representative lot was used in IH.

生物信息
免疫原品种	Phospho specific peptide corresponding to human Ezh2 phosphorylated at Thr487.
表位	Phosphorylated Thr487
克隆	EPR1410
宿主	Rabbit
特异性	This antibody recognizes Ezh2 phosphorylated at Thr487.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with Human, Mouse and Rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	Q15910
基因符号	EC 2.1.1 ENX-1 KMT6 EZH1 KMT6A EC 2.1.1.43 ENX1 MGC916 OTTHUMP00000213774 OTTHUMP00000213778 OTTHUMP00000213779 OTTHUMP00000213780
修改	Phosphorylation
纯化方法	Unpurified
UniProt编号	Q15910
UniProt汇总	FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. SUBUNIT STRUCTURE: Binds ATRX via the SET domain Probable. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICITY: Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. DEVELOPMENTAL STAGE: Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. INDUCTION: Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of p53/TP53. PTM: Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain. CAUTION: Two variants of the PRC2 complex have been described, termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms, although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2

产品使用声明
质量保证	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: A 1:1,000-1:10,000 dilution of this antibody detected Ezh2 in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.