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merck millipore,默克密理博,MABN691,Anti-GPI Antibody, clone 1B7D7
产品名称：Anti-GPI Antibody, clone 1B7D7
产品型号：MABN691
Anti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence.
merck millipore,默克密理博,MABN691,Anti-GPI Antibody, clone 1B7D7
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H IHC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 MABN691 描述 Anti-GPI Antibody, clone 1B7D7 Alternate Names - Sperm antigen 36
- Glucose-6-phosphate isomerase
- Autocrine motility factor
- Neuroleukin
- Phosphoglucose isomerase
- Phosphohexose isomerase
背景信息 Glucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by anon-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation. EMD-Millipore’s Anti-Glucose-6-phosphate isomerase monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HepG2, SMMC-7721 and rat liver tissue and in paraffin embedded immunohistochemistry on human kidney and fluorescent immunocytochemistry on L-O2 cells in culture. 产品信息 格式 Ascites 控制 - HepG2, SMMC-7721, and rat liver lysates
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Anti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in human kidney tissue.
Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in L-02 cells.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified recombinant fragment of human GPI expressed in E. Coli. 克隆 1B7D7 宿主 Mouse 同种型 IgG1 品种反应性 RatHuman 抗体类型 Monoclonal Antibody 基因符号 - GPI
- AMF
- NLK
- PGI
- PHI
- GNPI
- SA-36
纯化方法 Unpurified UniProt编号 - P06744
产品使用声明 质量保证 Evaluated by Western Blotting in HepG2, SMMC-7721, and rat liver lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPI in HepG2, SMMC-7721, and rat liver lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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