- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MABE57,Anti-E2F-6 Antibody, clone LL6-2
产品名称：Anti-E2F-6 Antibody, clone LL6-2
产品型号：MABE57
Use Anti-E2F-6 Antibody, clone LL6-2 (Mouse Monoclonal Antibody) validated in WB, IP to detect E2F-6 also known as E2F transcription factor 6.
merck millipore,默克密理博,MABE57,Anti-E2F-6 Antibody, clone LL6-2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ChIP, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE57 描述 Anti-E2F-6 Antibody, clone LL6-2 Alternate Names - E2F transcription factor 6, isoform 1
- E2F transcription factor 6
背景信息 The E2F family of transcription factors includes the E2Fs and DP1. These proteins are critical regulators of genes required for cell cycle progression, and their activity is regulated by association with different protein partners during stages of the cell cycle. E2Fs bind the hypophosphorylated form of pRb, and are maintained in an inactive state. Phosphorylation of Rb causes dissociation, and activation of E2F activity.
产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-E2F-6 Antibody, clone LL6-2 (Mouse Monoclonal Antibody) validated in WB, IP to detect E2F-6 also known as E2F transcription factor 6. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect E2F-6 in mouse embryonic stem cells. (Courel, M., et al. (2008). Developmental Dynamics. 237:1232–1242.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Trimarchi, J., et al. (2001). PNAS. 98(4):1519–1524.)生物信息 免疫原品种 Histidine-tagged recombinant protein corresponding to human E2F-6. 表位 Unknown 克隆 LL6-2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes E2F-6. 同种型 IgG2bκ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_937987
Entrez基因汇总 This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq]. 基因符号 - E2F6
- E2F-6
纯化方法 Protein G purfied UniProt编号 - O75461
UniProt汇总 FUNCTION: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
SUBUNIT STRUCTURE: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
SEQUENCE SIMILARITIES: Belongs to the E2F/DP family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected E2F-6 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,236600,C-Reactive Protein, Human Ascites
下一件merck millipore产品：merck millipore,默克密理博,344300,sFRP-1 Inhibitor - CAS 915754-88-0 - Calbiochem
相关产品
merck millipore,默克密理博,AB706-K,Goat Anti-Fibrinogen (1mL)
merck millipore,默克密理博,196876,BAY 41-2272 - CAS 256376-24-6 - Calbiochem
merck millipore,默克密理博,AQ127P,Goat Anti-Mouse IgG Antibody, Fc, HRP conjugate
merck millipore,默克密理博,09-213,Anti-phospho-mTOR (Ser2448) Antibody
merck millipore,默克密理博,236600,C-Reactive Protein, Human Ascites
merck millipore,默克密理博,344300,sFRP-1 Inhibitor - CAS 915754-88-0 - Calbiochem
merck millipore,默克密理博,573115,SMCC, Water-Soluble
merck millipore,默克密理博,203195,Protein A, Biotin Conjugate
merck millipore,默克密理博,SF-734,FLT3; Human, Cyanine 3, RNA Detection Probe,SmartFlare