merck millipore,默克密理博,MABE16,Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal

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merck millipore,默克密理博,MABE16,Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal
产品名称：Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal
产品型号：MABE16
Use Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y (Rabbit Monoclonal Antibody) validated in WB, IHC, ICC to detect phospho Sir2/SIRT1 (Ser47) also known as SIR2-like protein 1 or sirtuin 1.

merck millipore,默克密理博,MABE16,Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABE16,Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB, IHC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABE16
描述	Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y, rabbit monoclonal
Alternate Names	sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1 SIR2-like protein 1 sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
背景信息	The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2, SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

产品信息
格式	Unpurified
控制	Lambda phosphatase (LP)-treated and untreated 293 cell lysates.
演示	Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Use Anti-phospho Sir2/SIRT1 (Ser47) Antibody, clone EPR2849Y (Rabbit Monoclonal Antibody) validated in WB, IHC, ICC to detect phospho Sir2/SIRT1 (Ser47) also known as SIR2-like protein 1 or sirtuin 1.
主要应用	Immunocytochemistry Western Blotting Immunohistochemistry
应用说明	Immunohistochemistry Analysis: A 1:250 – 1:500 dilution from a previous lot detected Sir2/SIRT1 in IH. Immunocytochemistry Analysis: A 1:100 – 1:250 dilution from a previous lot detected Sir2/SIRT1 in IC.

生物信息
免疫原品种	Phospho-specific peptide corresponding to human Sir2/SIRT1 at Ser47.
表位	Ser47
克隆	EPR2849Y
宿主	Rabbit
特异性	This antibody detects Sir2/SIRT1 phosphorylated on Ser47.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_036370.2
基因符号	hSIRT1 sirtuin 1 SIR2L1 hSIR2
修改	Phosphorylation
纯化方法	Unpurified
UniProt编号	Q96EB6
UniProt汇总	FUNCTION: NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD+/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit By similarity. ENZYME REGULATION: Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity. Negatively regulated by KIAA1967/DBC1. SUBUNIT STRUCTURE: Interacts with TAF1B. Found in a complex with PCAF and MYOD1 By similarity. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML. Interacts with RPS19BP1/AROS. Interacts with KIAA1967/DBC1 (via N-terminus). SUBCELLULAR LOCATION: Nucleus › PML body. Note: Recruited to the nuclear bodies via its interaction with PML. TISSUE SPECIFICITY: Widely expressed. MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast. SEQUENCE SIMILARITIES: Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain. SEQUENCE CAUTIONS: The sequence AAH12499.1 differs from that shown. Reason: Erroneous initiation.

产品使用声明
质量保证	Evaluated by Western Blot in LP treated and untreated 293 cell lysate. Western Blot Analysis: A 1:1,000 – 1:2,000 dilution of this antibody detected Sir2/SIRT1 in LP treated and untreated 293 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.