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merck millipore,默克密理博,MABD102,Anti-Lin28 Antibody, clone PR3G10
产品名称：Anti-Lin28 Antibody, clone PR3G10
产品型号：MABD102
Anti-Lin28 Antibody, clone PR3G10 is a highly specific mouse monoclonal antibody, that targets lin28 & has been tested in western blotting & ICC.
merck millipore,默克密理博,MABD102,Anti-Lin28 Antibody, clone PR3G10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABD102 描述 Anti-Lin28 Antibody, clone PR3G10 Alternate Names - Zinc finger CCHC domain-containing protein 1
- Protein lin-28 homolog A
- Lin-28A
背景信息 Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells. 产品信息 格式 Purified 控制 - Human placenta tissue lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Anti-Lin28 Antibody, clone PR3G10 is a highly specific mouse monoclonal antibody, that targets lin28 & has been tested in western blotting & ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: 1 µg/mL from a representative lot detected Lin28 in human fetal brain tissue lysate.
Immunocytochemistry Analysis: A 1:500 dilution (2 µg/mL) from a representative lot detected Lin28 in mESCs.生物信息 免疫原品种 Ovalbumin-conjugated linear peptide corresponding to human Lin28. 表位 Near N-Terminus 克隆 PR3G10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_078950
基因符号 - ZCCHC1
- LIN28A
- CSDD1
- LIN28
纯化方法 Protein G Purified UniProt编号 - Q9H9Z2
UniProt汇总 FUNCTION: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus > nucleolus. Note: Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes. Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
TISSUE SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis.产品使用声明 质量保证 Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Lin28 in 10 µg of human placenta tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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